急性白血病流式细胞术免疫分型中系相关抗体的敏感性和特异性分析  

Sensitivity and Specificity Analysis of the Lineage Related Antibodies in Acute Leukemia Immunophenotyping by Flow Cytometry

作　　者：陈葆国[1] 张丽[2] 李伯利[1] 罗文达[2] 

机构地区：[1]温州医学院附属台州医院中心实验室,台州317000 [2]温州医学院附属台州医院 血液肿瘤内科,台州317000

出　　处：《中国实验血液学杂志》2005年第2期329-331,共3页Journal of Experimental Hematology

摘　　要：为了评估急性白血病流式细胞术免疫分型中系相关抗体的敏感性和特异性,参考St Jude研究医院的急 性白血病免疫分型方案对184例急性白血病患者进行流式细胞术免疫分型,结果表明:AML系相关抗体CD13和 CD33的敏感性较高(95.5％和91.2％),特异性不足(72.5％和62.2％);MPO敏感性低(69.1％),特异性高 (100％);CD117敏感性、特异性都高(88.2％和100％)。CD14、CD15敏感性低(18.4％和27.2％),CD14对单核细 胞特异性高。B细胞系ALL系相关抗体CD19具有高敏感性(100％),而特异性(83.4％)差;CD79a、CD22 (CyCD22)敏感性(96.4％和100％)和特异性(100％和100％)都高;CD10和CD20的敏感性(53.6％和70.4％)、 特异性(82.5％和87.5％)均低。T-ALL系列相关抗体CD3(CyCD3)高特异性(97.5％),而敏感性(80.0％)欠佳; CD7敏感性高(100％),特异性低(77.9％);CD5、CD2、CD1a敏感性、特异性均不理想。结论:184例急性白血病系 相关抗体的敏感性和特异性分析与St Jude免疫分型方案基本一致,只是在确定AML时CD117似乎优于MPO;确 定B细胞系ALL时CD22和CD79a具有相似的敏感性和特异性,所以可以任选一个。To evalaute the sensitivity and specificity analysis of the lineage related antibodies in acute leukemia immunophenotyping by flow cytometry (FCM), immunophenotyping in 184 patients with acute leukemia was performed by FCM analysis. The results showed that in the lineage-related antibodies of acute myelocytic leukemia ( AML) , the sensitivity of CD13 and CD33 was higher (95. 5% and 91. 2% , respectively), the specificity of them was deficient (72.5% and 62. 2% , respectively); the sensitivity of MPO was low (69. 1 % ), but the specificity was high (100% ); the sensitivity and specificity of CD117 were high (88.2% and 100% , respectively); the sensitivity of CD14 and CD15 was low (18.4% and 27. 2% , respectively); the specificity of CD 14 with monocytes was high. As the lineage-related antibodies of B-lineage ALL were concerned, CD19 showed high sensitivity and low specificity (100% vs 83.4% ); the sensitivity and specificity of CD79a (96. 4% vs 100% ) and CD22 ( 100% vs 100% ) were high; the sensitivity and specificity of CD10 (53.6% vs 82. 5%) and CD20 (70.4% vs 87. 5% ) were low. In T-lineage ALL, the specificity of CD3 was high (97.5% ), but the sensitivity was below the mark (80. 0% ); the sensitivity of CD7 was high (100% ), but the specificity was low (77.9% ); while the sensitivity and specificity of CD5,CD2 and CD1a were all deficient. In conclusion, the sensitivity and specificity analysis of the lineage-related antibodies in acute leukemia immunophenotyping are coincident with St Jude immunophenotyping project . It seems only that CD117 is superier to MPO in definiting AML, but the sensitivity and specificity analysis of CD22 and CD79 are similar in definiting B-lineage ALL, therefore, anyone of them may be selected as your need.

关 键 词：急性白血病 系列相关抗体 抗体敏感性 抗体特异性 

分 类 号：R733.71[医药卫生—肿瘤] R730.4[医药卫生—临床医学]