IL-15联合GM-CSF对急性白血病NK细胞作用的研究  

Study on activation of leukemia NK cell by IL15 and GM-CSF

作　　者：曹星梅[1] 王欢[1] 张王刚[1] 杨惠云[1] 田玮[1] 张慧[1] 何爱丽[1] 

机构地区：[1]西安交通大学第二医院,陕西西安710004

出　　处：《现代肿瘤医学》2005年第2期172-174,共3页Journal of Modern Oncology

基　　金：卫生部临床学科重点项目(No;2001S20)

摘　　要：目的　选择细胞因子IL-15和GM-CSF作为刺激物,观察其对急性白血病自然杀伤细胞(NK细胞)的激活作用。方法　分离健康成人及化疗缓解后的白血病患者外周血单个核细胞(PBMC),分别加入IL-2、IL-15、GM-CSF培养,MTT法检测PBMC及活化后的PBMC对白血病K562的细胞毒作用。流式细胞仪检测NK细胞并计数NK/PBMC比例。结果　正常人及化疗缓解组PBMC经过IL-15、IL-15+GM-CSF分别作用后,细胞均呈现不同程度的增殖。化疗后缓解的增殖程度均小于正常人。正常人及化疗缓解组PB MC联合应用IL-15和GM-CSF后对k562杀伤活性优于单用IL-15。缓解组对k562的杀伤活性明显低于正常对照组,化疗缓解后病人的NK/PBMC比例低于正常人。经联合应用IL-15、GM-CSF孵育后NK/PB MC高于正常人。结论　正常人及白血病患者PBMC与IL-15和GM-CSF联合孵育, 能有效刺激PBMC增殖,提高NK/PBMC的比例。通过正常人及白血病患者的PBMC对K562的杀伤能力,间接反应IL-15和GM-CSF能有效激活外周血NK细胞,增加杀伤活性。Objective Interleukin 15(IL-15), guanuloc yt e macrophage colony stimulating factor(GM-CSF) were used to activate NK cells. Methods Cells from normal adult peripheral blood and cells from complete remission (CR)leukemia patients peripheral blood were cultured b y IL-15 combination with GM-CSF. MTT methods were used to measure the proliferat ion of PBMC. Then, Cytotoxicity on leukemia cell line K562 mediated by PBMC acti vated by IL-15/GM-CSF were investigated. NK cells were identified on the basis o f surface phenotype by use of flow cytometry, then, the rate of NK/PBMC were cal culated. Results IL-15 combinaton with GM-CSF could induc e the proliferation of PBMC separated from healthy adults and CR patients, but t he proliferation of PBMC in CR patients was lower than healthy adults. Cytotoxic ity of PBMC in healthy adults stimulated with IL-15 alone was enhanced while cyt otoxicity of that with IL-15 combined GM-CSF was greater to IL-15 alone. Cytoto xicity of PBMC in CR patients stimulated with IL-15 and GM-CSF was enhanced. IL- 15 combinaton with GM-CSF increased effectively the rate of NK/PBMC in healthy adults. Compared with healthy controls ,the rate of NK/PBMC in CR patients was lower. Conclusions IL-15 and GM-CSF may induce the proli feration of PBMC in normal adults and CR patients and enhance its aytotoxicity t o K562. So, we think that IL-15 and GM-CSF may induce the proliferation of NK it s aytotoxicity, this maybe provide new methods for the acute leukemia immunother apy.

关 键 词：NK细胞 白血病 IL-15 GM—CSF 

分 类 号：R733.7[医药卫生—肿瘤]