人类神经干细胞系来源的肿瘤细胞系染色体G-显带比较分析  

Comparative analysis of G-banding in human neural stem cell derived tumor cell lines

作　　者：邹俊华[1] 韩玲[1] 刘卉[1] 

机构地区：[1]北京大学医学部医学遗传学系,100083

出　　处：《中华神经医学杂志》2005年第3期229-230,234,共3页Chinese Journal of Neuromedicine

摘　　要：目的比较人类神经干细胞源肿瘤细胞系和人类神经干细胞系染色体G-显带的差异,探讨神经干细胞成瘤性机制。方法制备人类神经干细胞系和其来源的肿瘤细胞系(T1和T2)细胞分裂中期染色体,胰酶处理后,经吉姆萨染色,用cytoscan-karyotyping FISH ﹠CGH 软件进行G-显带分析。结果人类神经干细胞源肿瘤细胞系染色体发生缺失、易位、双着丝粒等结构改变以及染色体数目改变,核型从46,XX到69,XX不等。结论人类神经干细胞来源的肿瘤细胞系染色体发生异常改变,表现为数量变化和结构重排。Objective To study the possible molecular mechanisms of tumorigenesis of the neural stem cell (NSC)-derived tumor which may occur in the NSC transplantation. Methods Chromosome analysis was performed by conventional Giemsa staining. Karyotyping was performed on the 5~8 passages of normal human NSCs (hNSCs), on the 5 passages of hNSC-derived tumor cells 6 weeks after hNSC transplantation into nude mice (T1) and tumor cells 15 weeks after transplantation (T2). Microscopic images of metaphases were acquired with a camera and a light microscope. The karyotypic analysis was performed with the cytoscan-karyotyping FISH ﹠ CGH software system. Results Complexity of chromosome abnormalities in hNSC-derived tumor cell lines is found to include deletion, translocation, dicentric and numerical changes. Karyotype changed from 46, XX to 69, XX in both tumor cell lines. Conclusion Chromosome abnormalities are preferentially involved in numerical and structural re-arrangements in the hNSC-derived tumor cell lines that derived from the same hNSC cell line. These chromosome changes suggest us to disclose intrinsic mechanisms of tumorigenesis of neural stem cells.

关 键 词：肿瘤细胞系 G-显带 干细胞系 人类神经 比较分析 神经干细胞来源 中期染色体 吉姆萨染色 染色体数目 细胞分裂 FISH 结构改变 异常改变 数量变化 细胞源 成瘤性 酶处理 CGH 着丝粒 

分 类 号：R73-3[医药卫生—肿瘤]