HPV16E6/E7融合蛋白疫苗与放疗联合应用治疗宫颈癌的实验研究  

The Therapeutic Effect of Combination of the Human Papillomavirus 16 ( HPV16) E6/E7 Fusion Protein Vaccine Combined with Radiotherapy on Cervical Cancer

作　　者：周小山[1] 千新来[1] 陆元志[1] 刘巧[1] 赵清正[1] 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所生物化学与分子生物学实验室,北京100021

出　　处：《中国肿瘤生物治疗杂志》2005年第1期19-22,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家"863"计划项目(NO:2002AA216041)

摘　　要：目的:观察人乳头瘤病毒16型(HPV)E6/E7融合蛋白疫苗与放疗(12Gy)联合应用对宫颈癌的治疗效果。方法:24只雌性C57BL/6小鼠右侧后肢接种肿瘤细胞后7d,随机分为4组:对照组(C,n=6);免疫组(IM,n=6);放疗组(RA,n=6);联合治疗组(IM+RA,n=6)。比较各组小鼠的肿瘤生长速度及存活时间。采用TUNEL法对小鼠肿瘤组织中细胞的凋亡情况进行检测。结果:IM+RA组小鼠肿瘤生长速度较慢,平均存活时间明显长于RA、IM及C组,IM组与C组动物的平均存活时间没有差异。TUNEL实验结果表明,IM+RA组小鼠肿瘤组织中凋亡细胞明显高于其他各组。结论:HPV16E6/E7融合蛋白疫苗与放疗联合应用,具有协同抗肿瘤作用。Objective: To evaluate the therapeutic effect of combination of the human papillomavirus 16 (HPV16) E6/ E7 fusion protein vaccine with radiotherapy on the HPV16-positive cervical cancer. Methods: Twenty-four female C57BL/6 mice 7 days after inoculation of tumor cells on the right hind legs were randomly divided into four groups: The control group ( C, n = 6 ) ; Immunotherapy group (IM , n = 6 ) ; Radiotherapy group ( RA, n = 6 ) ; Immunotherapy + Radiotherapy (IM + RA, n = 6 ) . The growth rates of tumors and survival time were recorded and compared with each other. The apoptotic tumor cells in situ were detected by TUNEL assay. Results: The growth rates of tumors in IM + RA- group were lower than other groups. The mean survival time in IM + RA-group was longer than those in IM-, RA- and C-groups, but the difference between IM- and C- group was not significant. The result of TUNEL assay indicated that there were more apoptotic tumor cells in IM + RA- group than that in other groups. Conclusion: The combination of HPV16E6/E7 fusion protein vaccine with low dose radiotherapy exerted synergic antitumor efficacy, which might provide a new approach for the treatment of cervical cancer.

关 键 词：人乳头瘤病毒16型 免疫治疗 放疗 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]