染料木素对去卵巢大鼠脑突触体[Ca^(2+)]_i及膜流动性的影响  

THE INFLUENCES OF GENISTEIN ON SYNAPTOSOME MEMBRANE FLUIDITY AND INTRASYNAPTOSOME CALCIUM IN OVARIECTATIZED RATS'BRAIN

作　　者：朱建华[1] 郭开华[1] 徐杰[1] 

机构地区：[1]中山大学中山医学院解剖学教研室,广州510080

出　　处：《解剖学报》2005年第2期131-134,共4页Acta Anatomica Sinica

基　　金：广东省自然科学基金重点项目(4105332)资助

摘　　要：目的　观察去卵巢大鼠大脑皮质和海马突触体[Ca2+]i和膜流动性变化以及染料木素的治疗作用。 　方法　雌性SD大鼠随机分为假手术组、去卵巢对照组、染料木素组、苯甲酸雌二醇组。分别皮下注射生理盐水 50μ1、生理盐水50μ1、染料木素250μg和苯甲酸雌二醇50μg,隔日1次。术后8周测定各组大鼠大脑额、顶叶皮质 和海马的突触体[Ca2+]i及膜流动性。　结果　去卵巢对照组大鼠大脑皮质及海马突触体[Ca2+]i分别为(243.31 ±31.21)nmol/L和(305.10±54.31)nmol/L,与假手术组、苯甲酸雌二醇组和染料木素组之间有显著性差异(P< 0.01);但在假手术组、苯甲酸雌二醇组和染料木素组之间无显著差异(P>0.05);去卵巢对照组海马突触体膜相对 黏滞度(η)为3.03±0.39,与假手术组和苯甲酸雌二醇组之间有显著性差异(P<0.05);与染料木素组相比有高度 显著性差异(P<0.01)。染料木素组、苯甲酸雌二醇组及假手术组之间无显著差异(P>0.05)。　结论　大鼠去卵 巢后突触体膜流动性降低及突触体内自由钙增加,染料木素能增加去卵巢大鼠突触体膜流动性及维持钙稳态。Objective To observe the changes of synaptosome membrane fluidity and intrasynaptosome free calcium in ovariectomized rats' brain and the therapeutical effects of genistein. Methods Thirty-six 3 month-old female Sprague-Dawley rats were randomly assigned to 4 groups:sham-operated,ovariectomized control,genistein and estradiol benzoate groups.Normal saline(50?μl),normal saline(50?μl),genistein(250?μg)and estradiol benzoate (50?μg)were subcutaneously injected respectively once every other day for 8 weeks,and then synaptosome membrane fluidity and intrasynaptosome free calcium in frontal and parietal lobe and hippocampus were detected. Results The intrasynaptosome free calcium of cerebral cortex and hippocampus synaptosomes of ovariectomized control group (±s)are (243.31±31.21)nmol/L and (305.10±54.31)nmol/L respectively.There are significantly statistical differences as compared ovariectomized control group with sham-operated,genistein and estradiol benzoate groups(P<0.01),but no statistical differences among sham-operated,genistein and estradiol benzoate groups were seen(P>0.05).The synaptosome membrane viscosity(η)of hippocampal synaptosomes of ovariectomized control group(±s)is 3.03±0.39,which has significantly statistical differences compared with sham-operated and estradiol benzoate groups(P<0.05),and compared with genistein group (P<0.01),but no statistical differences among sham-operated,genistein and estradiol benzoate groups(P>0.05).Conclusion The synaptosome membrane fluidity decreased while intrasynaptosome free calcium increased in ovariectomized rats' synaptosomes.Genistein can enhance the membrane fluidity and maintain intrasynaptosome free calcium in ovariectomized rats' synaptosomes.

关 键 词：染料木素 钙稳态 膜流动性 神经退行性病 大鼠 

分 类 号：Q189[生物学—神经生物学] R741.02[生物学—普通生物学]