铁离子对Caco-2细胞内钙离子转运的影响及钙离子浓度变化与细胞凋亡关系的研究  

THE INFLUENCE OF Fe^(3+) ON Ca^(2+) AND THE RELATIONSHIP BETWEEN Ca^(3+) AND THE CELL APOPTOSIS IN Caco-2 CELLS

作　　者：王莉[1] 常彦忠[1] 段相林[1] 

机构地区：[1]河北师范大学生命科学学院动物细胞生物学研究室,石家庄050016

出　　处：《解剖学报》2005年第2期177-181,共5页Acta Anatomica Sinica

基　　金：自然科学基金资助项目(省303158);河北省教育厅基金课题(2002136)

摘　　要：目的　研究细胞外铁离子(Fe3+)浓度变化对钙离子(Caco 2)细胞内Ca2+转运的影响及细胞内钙离子 浓度升高与Caco 2细胞凋亡的关系。　方法　采用激光共聚焦扫描显微镜和流式细胞技术进行观察与检测。　 结果　(1)利用激光共聚焦扫描显微镜(CLSM)观察发现,随着Caco 2细胞外Fe3+浓度的减少(DFO终浓度为100～ 300μmol L),Ca2+向细胞内的转运量增加;而随着细胞外Fe3+浓度的增加(FAC终浓度为10～100μmol L),Ca2+向细 胞内的转运呈降低趋势;(2)经A23187和Fluo 3 AM处理后Caco 2细胞的存活率较高,达90%以上(用荧光素二醋酸 酯检测);(3)A23187升高Caco 2细胞的胞内Ca2+浓度,呈剂量依赖性;经流式细胞技术(FCM)检测发现胞内Ca2+浓度 增加具有诱导Caco 2细胞凋亡的作用。　结论　Caco 2细胞外Fe3+浓度的减少有促进Ca2+向细胞内转运的作用, 但胞外Fe3+浓度增加时有抑制Ca2+向胞内转运的作用;胞内Ca2+浓度增加对Caco 2细胞凋亡具有诱导作用。Objective To study the influence of ion concentration change out of cells on calcium transportation and the relationship between the rising of calcium concentration in the Caco-2 cells and its apoptosis to offer the theoretical and experimental bases for clinical study and digestive tract physiology and patholoogy. Methods Confocal laser scanning microscope(CLSM) and flow cytometry(FCM) were used in this study. Results (1) Diversion of Ca 2+ into cells was increased with the decrease of Fe 3+ concentration out of Caco-2 cells(the final consistence of DFO was 100-300?μmol/L),but was hindered with the increase of Fe 3+ concentration out of them(the final consistence fo FAC was 10-100?μmol/L) as observed under CLSM; (2) The cell state was fine and its viability was more than 90% as observed under CLSM after treated by A 23187 and Fluo-3/AM(examined with FDA);(3) The Ca 2+ concentration in the Caco-2 cells was increased by A 23187 and this function was dose depended.The Caco-2 cell apoptosis was induced by the increase of Ca 2+ in cells,which was examined with FCM.Conclusion The Ca 2+ transportation was increased with the decrease of Fe 3+ concentration out of Caco-2 cells but was hindered with the increase of Fe 2+ concentration out of them.The Caco-2 cell apoptosis was induced by the increase of Ca 2+ concentration in them.

关 键 词：细胞凋亡 浓度变化 钙离子转运 激光共聚焦扫描显微镜 Caco-2细胞 CA^2+浓度 离子对 细胞内钙离子浓度 流式细胞技术 CA^2+转运 A23187 Fluo-3 剂量依赖性 细胞内转运 细胞外 诱导作用 铁离子 检测 存活率 醋酸酯 荧光素 

分 类 号：R735.7[医药卫生—肿瘤] R459.5[医药卫生—临床医学]