食管鳞癌△Np63基因的检测  

△Np63 gene expression in esophageal squamous cancer

作　　者：马军[1] 李印[2] 段芳龄[1] 郑世营[3] 虎建恩[1] 郭敏 

机构地区：[1]郑州大学消化疾病研究所,河南郑州450014 [2]河南省肿瘤医院胸外科 [3]苏州大学第一医院胸心外科 [4]罗彻斯特大学医学中心放射和癌症系,美国罗彻斯特市14642

出　　处：《第四军医大学学报》2005年第7期640-642,共3页Journal of the Fourth Military Medical University

摘　　要：目的: 探讨△Np63基因作为食管鳞癌检测指标的可能性. 方法: 50例新鲜食管鳞癌组织,未经放疗或化疗,癌旁组织15例,用免疫组化和RT PCR方法检测组织和外周血△Np63表达情况. 食管癌患者外周血标本21例,用RT PCR方法检测了△Np63mRNA表达情况,胃腺癌12例和10例健康人外周血作为对照. 结果: 食管鳞癌组织△Np63蛋白和mRNA表达明显高于癌旁组织,分别是96%和60% (P<0. 01). 食管癌21例外周血检测中△Np63 mRNA阳性率38%,而胃腺癌和健康人外周血中未见表达. 结论: 食管鳞癌的发生和△Np63过度表达有关,△Np63mRNA外周血检测可以作为一个食管鳞癌的肿瘤标志物.AIM: To detect the expression of △Np63 gene in esophageal squamous cancer. METHODS: Fifty fresh tissue specimens as well as 21 peripheral blood samples of esophageal squamous cancer without chemotherapy and radiation, 15 para-cancerous tissue specimens, 12 peripheral blood samples of gastric adenocarcinoma and 10 peripheral blood samples of healthy volunteer were used to detect △Np63 gene expression using immunohistochemistry and RT-PCR. RESULTS: △Np63 gene expression in cancer tissues was up-regulated compared with that in para-cancerous tissue at both mRNA and protein levels (96% and 60% respectively, P <0.01). △Np63 mRNA was detected in 38% of the peripheral blood samples in esophageal squamous cancer but not in gastric adenocarcinoma and healthy volunteer. CONCLUSION: △Np63 mRNA of peripheral blood can be used as a tumor marker of esophageal squamous cancer.

关 键 词：食管鳞癌 ANp63基因 

分 类 号：R735[医药卫生—肿瘤]