p16^(INK4A)诱导的衰老相关基因表达差异  

DOFFEREMTIAL EXPRESSION OF CELLULAR SENESCENCE-ASSOCIATED GENES INDUCED BY p16 INK4A

作　　者：韩晓琳[1] 

机构地区：[1]济宁医学院生物化学教研室

出　　处：《济宁医学院学报》2005年第1期3-6,共4页Journal of Jining Medical University

摘　　要：目的　检测衰老主导基因 p16与衰老相关基因 p2 1、CSIG、TOM 1间的相互关系。 方法　正、反义 p16转染人胚肺 2倍体成纤维细胞 (2BS)后 ,用RT -PCR检测 p2 1、CSIG、TOM 1的表达 ,用WesternBlot检测 p2 1蛋白的表达。 结果　p16对p2 1存在转录后调控作用 ,正义 p16转染细胞TOM 1高表达 ,反义p16转染细胞CSIG高表达。 结论　衰老主导基因 p16与衰老相关基因p2 1、CSIG、TOM 1间存在相互作用 ,进一步说明了 p16在衰老中的主导地位。Objective To explore the relationship betweensenescence-associated Genes of p16 and p21、CSIG、TOM1.Methods The constructed retroviral vectors pDOR-p16 (sense p16) and pDOR-asp16 (anti-sense p16) were transfected into Human diploid fibroblasts (2BS) cells,the expression levels of p21,CSIG and TOM1 mRNA were examined by RT-PCR and also were examined by Western Blot the expression levels of p21 protein.Results The result showed that p16 regulate p21 via a posttranscriptional mechanisms.highexpression of TOM1 was found in cells transfectd by sense p16,and so of CSIG in cells transfected by anti-sense p16.Conclusion Senescence-associated genes of p16 and p21,CSIG and TOM1 affect each other which give evidence that p16 play a major role in cell senescence.

关 键 词：P16^INK4A 基因表达差异 衰老 相关 RT-PCR检测 Western 基因p21 基因P16 BLOT检测 转染细胞 成纤维细胞 P21蛋白 相互关系 调控作用 相互作用 主导地位 高表达 人胚肺 

分 类 号：R730.2[医药卫生—肿瘤] R992[医药卫生—临床医学]