基于尼古丁对胆碱氧化酶的抑制作用测定尼古丁的研究  

Determination of Nicotine Based on the Inhibitionof Nicotine to the Choline Oxidase

作　　者：阳明辉[1] 杨云慧[1] 沈国励[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《分析化学》2005年第4期479-482,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No. 20075006;20375012; 20205004);博士点基金(No. 20010532008)资助项目

摘　　要：基于尼古丁对胆碱氧化酶 (CHOD) 的抑制作用,将胆碱氧化酶电极用于微量尼古丁的测定.酶电极制作中,先在铂电极表面修饰一层壳聚糖膜,再用戊二醛交联CHOD.采用这种酶固定化方法,电极在4.0×10-6～ 3×10-3 mol/L 胆碱的浓度范围呈线性关系.探讨了工作电位、pH、底物浓度等实验条件对酶电极性能及抑制过程中响应电流的影响,测定了电极的重现性、干扰及使用寿命.电极检测尼古丁的线性范围为1.5×10-5～3×10-3 mol/L;检出限为1.25×10-5 mol/L.将电极用于实际样品烟草中尼古丁回收率的测定,结果良好.Based on the inhibition of nicotine to the choline oxidase(CHOD), the CHOD electrode has been used to the determination of nicotine. For the fabrication of CHOD electrode, a chitosan layer was first formed onto the surface of the Pt electrode, then CHOD was immobilized onto the electrode through glutaraldehyde. Using this kind of immobilization method, the electrode exhibits a linear response range to choline from 4. 0 x 10(-6) ∼ 3 x 10(-3) mol/L. The effects of experimental conditions such as applied potential, pH, substrate concentration on the electrode performance and response current in the inhibition were discussed. The linear range of detection of the electrode to nicotine is 1. 5 x 10 (-5) ∼ 3 x 10 (-3) mol/L, with detection limit 1. 25 x 10(-5) mol/L. The electrode was applied to the determination of the recoveries in tobacco sample with satisfactory results.

关 键 词：胆碱氧化酶 抑制作用 尼古丁 测定 氧化酶电极 固定化方法 壳聚糖膜 表面修饰 线性关系 浓度范围 工作电位 响应电流 电极性能 实验条件 底物浓度 使用寿命 线性范围 铂电极 戊二醛 重现性 检出限 回收率 

分 类 号：O657.1[理学—分析化学]