脑星形细胞瘤端粒酶活性及其临床意义  

Telomerase activity in astrocytoma and its clinical significance

作　　者：常会民[1] 谭平国[2] 

机构地区：[1]武警广东总队医院神经外科,广州510507 [2]中山大学第二临床学院神经外科,广州510120

出　　处：《武警医学》2005年第3期182-184,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的　检测脑星形细胞瘤组织中端粒酶的活性 ,分析其活性变化在肿瘤不同病理类型之间的关系。方法　选用 76例I～IV级脑星形细胞瘤患者的手术切除标本作为实验标本 ;应用端粒重复扩增法 (TRAP -ELISA)分别定量检测不同病理类型星形细胞瘤组织中端粒酶的活性。另取 1 5例正常脑组织标本作为对照。结果　 76例脑星形细胞瘤患者中 ,Ⅰ～Ⅱ级者 38例 ,端粒酶阳性者 3例 (7.9%) ;星形细胞瘤Ⅲ～Ⅳ级者 38例 ,端粒酶阳性者 33例 (86 .6 %)。两组端粒酶阳性率之间有显著 (P <0 .0 1 ) ;两组之间 ,端粒酶活性定量水平之间也有显著性差异 (P <0 .0 5 )。 1 5例正常脑组织中未检测到端粒酶活性。结论　端粒酶的活性在脑星形细胞瘤中随着肿瘤活性程度的增高而增加。说明端粒酶作为肿瘤标志物在星形细胞瘤良恶性鉴别、恶性程度评估方面具有重要意义。Objective To investigate the telomerase activity in astrocytoma and its relationship to different histopathological grades of the tumor.Methods Seventy-six tumor samples taken from patients with astrocytoma were used to detect the telomerase activity by TRAP-ELISA method. Another 15 normal brain tissues were employed as the controls.Result In 76 astrocytomas,3 of 38 (7.6%) low-grade astrocytoma(grades Ⅰ-Ⅱ)were detected to be telomerase positive;33 of 38 (86.6%) high-grade astrocytoma (grades Ⅲ-Ⅳ) were positive in telomerase expresson. The positive rates in these two groups were significantly different(P<0.001),and their levels of telomerase activity were also significantly different(P<0.05). The telomerase activity was not detected in 15 normal brain tissues.Conclusion The telomerase activity in astrocytoma increases with advancing histopathological grade of the tumor,which is of- significant importance in differentiating between benign and malignant tumors,and evaluating malignancy of tumors.

关 键 词：脑星形细胞瘤 端粒酶活性 临床意义 端粒重复扩增法 手术切除标本 病理类型 显著性差异 正常脑组织 良恶性鉴别 肿瘤标志物 活性变化 实验标本 定量检测 组织标本 重要意义 恶性程度 瘤组织 阳性者 阳性率 瘤活性 患者 

分 类 号：R739.41[医药卫生—肿瘤] R734.2[医药卫生—临床医学]