CXCL16在小鼠免疫性肝损伤中的作用及意义  

The role of CXCL16 in immunological liver injury induced by BCG and LPS in mice

作　　者：徐焕宾[1] 龚燕萍[1] 蒋正刚[1] 刘瑞梓[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫系,教育部分子医学重点实验室,上海200032

出　　处：《中华肝脏病杂志》2005年第4期282-285,共4页Chinese Journal of Hepatology

基　　金：国家教育部科学技术重点项目(03063)国家杰 出青年科学基金(39925031)国家重点基础研究发展计划 (2001CB510005)

摘　　要：目的研究CXCL16的表达对小鼠免疫性肝损伤中的作用及意义。方法建立卡介苗和内毒素诱导的小鼠肝损伤模型,通过实时定量聚合酶链反应和免疫组织化学分析CXCL16在肝组织中的表达变化,根据肝脏病理变化和免疫组织化学结果分析,比较CXCL16的表达水平和肝脏损伤程度的关系。从模型中各个时间点的肝损伤组织中分离浸润单核细胞,计算浸润的细胞数量变化,并进一步分析其中主要的CD4和CD8T淋巴细胞亚群的数量变化,研究CXCL16在肝脏炎症和损伤中的作用和意义。结果成功复制了小鼠免疫性肝损伤模型,发现在肝脏炎症和损伤中CXCL16表达水平显著上调,CXCL16的表达水平与肝脏损伤程度密切相关,肝脏组织浸润的单核细胞数量明显增加,淋巴细胞尤其是CD4+、CD8+T淋巴细胞数量也有明显的增加。结论小鼠肝脏损伤过程中CXCL16表达水平的增加与肝损伤程度密切相关,CXCL16趋化淋巴细胞浸润可能是导致肝脏损伤的重要机制之一。Objective To investigate the pathophysiological role of CXCL16 in immunological liver injury induced by Bacille de Calmette et Guerin (BCG) and lipopolysaccharides (LPS). Methods Immunological liver injury was induced by BCG and LPS in mice, and the expression of CXCL16 was detected in the liver tissues by real-time quantitative PCR and immunohistochemical examination. The relationship of the expression of CXCL16 and the extent of hepatic necrosis was investigated histopathologically and immunohistochemically. Mononuclear cells were isolated from the liver tissues and their numbers were counted; T lymphocytes populations in the liver tissue were also analyzed with FACS. Results The immunological liver injury model was successfully created. Up-regulation of CXCL16 in injured livers correlated with the extent of liver injury and the amountmononuclear cell infiltrations. Conclusion These findings suggest that up-regulation of CXCL16 was closely correlated with liver injury extent during the immunological liver injury induced by BCG-LPS in mice, and intrahepatic recruitment of specific lymphocytes might be an important mechanism of liver injury.

关 键 词：CXCL16 小鼠 免疫性肝损伤 趋化因子类 淋巴细胞 

分 类 号：R575[医药卫生—消化系统]