肿瘤转移相关基因mts1及nm23-H1在肺癌中的表达及其与淋巴结转移的关系  

Tumor metastasis associated gene mts1 and nm23-H1: expression in the lung carcinoma and relationship with lymph node metastasis

作　　者：许建平[1] 谢青[1] 张哉根[1] 叶明福[1] 陈意生[2] 高广正[1] 

机构地区：[1]第三军医大学新桥医院病理科,重庆400037 [2]第三军医大学西南医院病理科,重庆400038

出　　处：《第三军医大学学报》2005年第7期655-657,共3页Journal of Third Military Medical University

摘　　要：目的　探讨转移相关基因mts1及nm2 3 H1在人肺癌中的表达及其与淋巴结转移的关系。方法　对手术切除后新鲜的2 6例肺鳞癌和13例肺腺癌组织标本采用地高辛标记的mts1及nm2 3 H1cDNA探针进行原位杂交。结果　2 6例鳞癌中,淋巴结转移组11例,mts1mRNA阳性表达9例,阳性率82 % ,nm2 3 H1mRNA阳性表达8例,阳性率73 % ;无淋巴结转移组15例,mts1mRNA阳性表达4例,阳性率2 7% ,nm2 3 H1mRNA阳性表达10例,阳性率67%。13例肺腺癌中,淋巴结转移组5例,mts1mRNA阳性表达4例,阳性率80 % ,nm2 3 H1mRNA阳性表达4例,阳性率80 % ;无淋巴结转移组8例,mts1mRNA阳性3例,阳性率3 8% ,nm2 3 H1mRNA阳性表达6例,阳性率75 %。结论　mts1mRNA表达在肺鳞癌的淋巴结转移与非转移组之间相差显著,在肺腺癌的淋巴结转移组与非转移组之间差异性无统计学意义;nm2 3 H1mRNA表达在肺鳞癌、腺癌的淋巴结转移组与非转移组之间差异性不显著。Objective To study the expression of tumor metastasis associated gene mts1 and nm23-H1 in the human lung carcinoma and their relationship with lymph node metastasis. Methods Fresh specimens excised from 26 cases of human lung squamous cell carcinoma and 13 cases of human lung adenocarcinoma were hybridized in situ with mts1 and nm23-H1 cDNA probes marked with digoxin. Results In 26 cases of human lung squamous cell carcinoma, there were 11 cases of lymph node metastasis, including 9 cases of positive mts1 mRNA expression (82%) and 8 cases of positive nm23-H1 mRNA expression (73%), and 15 cases of non-lymph node metastasis, including 4 cases of positive mts1 mRNA expression (27%) and 10 cases of positive nm23-H1 mRNA expression (67%). In 13 cases of human lung adenocarcinoma, there were 5 cases of lymph node metastasis, including 4 cases of positive mts1 mRNA expression (80%) and 4 cases of positive nm23-H1 mRNA expression (80%), and 8 cases of non-lymph node metastasis, including 3 cases of positive mts1 mRNA expression (38%) and 6 cases of positive nm23-H1 mRNA expression (75%). Conclusion mts1 mRNA expression in lung squamous carcinoma was significantly different between lymph node metastasis group and non-lymph node metastasis group and that in lung adenocarcinoma was of no difference between two groups. nm23-H mRNA expression in lung squamous carcinoma or in lung adenocarcinoma was of no significant difference between two groups.

关 键 词：MTS1基因 NM23-H1基因 肺癌 淋巴结转移 原位杂杂 

分 类 号：R394.2[医药卫生—医学遗传学] R73-37[医药卫生—基础医学]