EFFECTS OF SDS,PBS SOLUTIONS UPON FLUORESCENCE VALUES OF DERMATOPHAGOIDES PTERRONYSSINUS RADIOALLERGOSORBENT TEST INHIBITION  

作　　者：Jin-lüSun Hong-yuZhang Hai-juanHe Rui-qiWang 

机构地区：[1]DepartmentofAllergy,PekingUnionMedicalCollegeHospital,ChineseAcademyofMedicalSciences＆PekingUnionMedicalCollege,Beijing100730

出　　处：《Chinese Medical Sciences Journal》2005年第1期27-29,共3页中国医学科学杂志（英文版）

基　　金：Supported by a grantfrom the Ministryof Science and Technology PRC (2003AA2Z3502 ).

摘　　要：Objective To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition. Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12 505 and 24 776. Results Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively. Conclusions SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.

关 键 词：Dermatophagoides pterronyssinus radioallergosorbent test inhibition ALLERGEN 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]