人增殖抑制基因(HSG)对肿瘤细胞系化疗敏感性的作用  

Human hyperplasic suppress gene (hHSG) could increase the chemotherapy sensitivity of human tumor cells in vitro

作　　者：王萍[1] 毋丽娜[1] 蒋春笋[2] 李志新[1] 张颖妹[1] 陈光慧[1] 邱晓彦[1] 

机构地区：[1]北京大学人类疾病基因研究中心,北京100083 [2]中国科学院动物研究所

出　　处：《北京大学学报（医学版）》2005年第2期117-120,共4页Journal of Peking University:Health Sciences

基　　金：国家高技术研究发展计划专项经费 (2002AA216131 )资助~~

摘　　要：目的:将人增殖抑制基因 (hHSG)用电子穿孔的转基因方式转染到体外培养的人肿瘤细胞系中,观察hHSG基因对内源性hHSG表达水平不同的肿瘤细胞系的化疗敏感性的影响。方法:首先用免疫组化方法检测不同组织来源的肿瘤细胞系中hHSG的表达水平,然后选择内源性hHSG表达水平较低的肺腺癌 (A549 )和内源性hHSG表达水平较高的宫颈癌(HeLaS3)细胞系,用电穿孔方法转染含有hHSG的真核表达载体 (pEGFP hHSG) 24h后,加入放线菌酮(CHX),采用细胞计数、MTT法观察hHSG对肿瘤细胞增殖抑制作用及对CHX的化疗敏感性的影响。结果:hHSG在不同组织来源的肿瘤细胞系都有不同程度的表达, pEGFP hHSG转染到两种内源性hHSG表达水平不同的肿瘤细胞系后,这两种肿瘤细胞的生长增殖都明显受到抑制,同时外源性的hHSG也增强了这些肿瘤细胞系对CHX的敏感性。结论:外源性hHSG可不依赖其内源性表达水平而抑制肿瘤细胞的增殖,与CHX并用可增强肿瘤细胞对CHX的敏感性,具有协同作用。Objective: To investigate whether the hHSG can increase the chemotherapy sensitivity of two human tumor cell lines,lung cancer cell line (A549) and cervical cancer cell line (HeLa S3), in which the hHSG expression levels are different. Methods:After detecting the hHSG expression in different tumor cell lines with immunohistochemistry; we selected the A549 with relatively low expression of hHSG and HeLa S3 with high expression. After these two cell lines were transfected with recombinant eukaryotic expression vector of pEGFP-hHSG and pEGFP by electroporation respectively,and cultured for 24 h, the CHX was added to the medium,and the impacts of hHSG on chemotherapy sensitivity were evaluated by cell counting and MTT assay in the following days. Results:hHSG expressed at different levels in all investigated tumor cell lines. Exogenetic hHSG inhibited the proliferation of tumor cell lines, and increased their sensitivity for CHX significantly.Conclusion: hHSG gene can inhibit the proliferation of tumor cells and increase their chemotherapy sensitivity despite of its endogenetic expression levels.

关 键 词：化疗敏感性 抑制基因 人肿瘤细胞系 免疫组化方法 真核表达载体 增殖抑制作用 抑制肿瘤细胞 组织来源 内源性 CHX 体外培养 基因方式 放线菌酮 细胞计数 MTT法 不同程度 生长增殖 协同作用 外源性 水平 转染 G基因 HHS 

分 类 号：R730.53[医药卫生—肿瘤] R730.21[医药卫生—临床医学]