视网膜小胶质细胞活化模型的建立  

Establishment of a model of retinal microglial cells activation in vitro

作　　者：王爱玲[1] 柳林[2] 朱秀安[1] 曹安民[1] 

机构地区：[1]北京大学第三医院,北京大学眼科中心,北京100083 [2]北京大学医药卫生分析中心

出　　处：《北京大学学报（医学版）》2005年第2期198-200,共3页Journal of Peking University:Health Sciences

摘　　要：目的: 建立视网膜小胶质细胞培养、纯化和鉴定的方法,以观察视网膜小胶质细胞活化后的形态和功能变化,探讨活化的小胶质细胞在糖尿病视网膜病变时的可能作用。方法: 以细菌内毒素脂多糖 (LPS)活化小胶质细胞,通过免疫细胞化学及con focal显微镜技术、流式细胞术、MTT、ELISA等方法观察视网膜小胶质细胞形态、数量和功能的变化。结果: 视网膜小胶质细胞纯度在 96%以上,LPS激活的小胶质细胞发生形态改变,CD11b表达上调,释放细胞因子TNF α,而细胞数量无明显改变。结论: 从细胞免疫表型、纯度、形态学和分泌功能等方面都证明,本方法是一种稳定、高效的小胶质细胞分离纯化方法。Objective: To develop a method of retinal microglial cell culture to study the function of the microglial cell in diabetic retinopathy. Methods: Microglia were activated with LPS. Immunocytochemistry, con-focal microscopy, flow cytometry, MTT and ELISA were applied to observe the morphological characters, quantity, and functional changes of the microglia. Results: The purity of the microglia was up to 96% as determined by immunostaining and flow cytometry. Some morphological changes of microglia were observed after treatment with LPS, but their quantity kept stable. Cytokine TNF-α released from microglia increased significantly. Conclusion: The isolated microglial cells are pure by using this culture system, which would provide a valuable tool for studying mechanisms of microglial alterations in diabetic retinopathy.

关 键 词：小胶质细胞活化 糖尿病视网膜病变 脂多糖(LPS) 模型 免疫细胞化学 细胞免疫表型 分离纯化方法 细菌内毒素 流式细胞术 ELISA CD11B TNF-α 细胞培养 功能变化 细胞形态 形态改变 细胞发生 表达上调 细胞因子 细胞数量 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R587.2[医药卫生—临床医学]