前列腺癌PC-3M细胞hmSD活性与细胞凋亡相关性的实验研究  

Relation between hmSD activity and apoptosis rate in a PC-3M cell line

作　　者：李扬[1] 狄茜[1] 许莉[1] 刘喜春[1] 赵丹[1] 赵雪俭[1] 宫城妙子 

机构地区：[1]吉林大学基础医学院病理生理教研室,吉林长春130021 [2]日本宫城县癌中心

出　　处：《中国病理生理杂志》2005年第4期748-751,共4页Chinese Journal of Pathophysiology

基　　金：吉林省科技厅资助项目 (No.2 0 0 10 70 9)

摘　　要：目的:研究前列腺癌PC - 3M细胞株hmSD的活性与细胞凋亡的相关性。方法:应用丁酸钠诱导前列腺癌PC - 3M细胞发生凋亡,吖啶橙/溴化乙锭染色检测细胞凋亡率;收集丁酸钠作用后的PC - 3M细胞,匀浆后离心取上清,与底物共同孵育,以硫代巴比妥酸(TBA)显色反应检测所生成的唾液酸,计算唾液酸酶活性;将细胞凋亡率与hmSD活性进行相关性分析。结果:丁酸钠诱导PC - 3M细胞凋亡的同时显著下调hmSD的活性,凋亡率与hmSD活性呈负相关。结论:hmSD活性变化与前列腺癌细胞凋亡呈相关性。AIM: To investigate the correlation betwe en human membrane-associated sialidase (hmSD) activity and apoptosis rate in a PC- 3M cell line. METHODS: Sodium butyrate was added to the culture media of PC-3M cells for 48 h to induce apoptosis and then the cells were collected, homogenat e d and centrifuged. The supernatant was incubated with the substrate. TBA reactio n was then used to detect the sialic acid and the activity of the sialidase was calculated. Meanwhile, the apoptosis rate was detected by AO/EB assay, and the c orrelation between hmSD activity and apoptosis rate was calculated. RESULTS: HmSD activity of PC-3M cells was significantly down-reg ulated when cell apoptosis was induced by sodium butyrate and high correlation b etween hmSD activity and apoptosis rate was observed. CONCLUSION: There is a significant correlation between apoptosis rate and hmSD activity in prostate cancer.

关 键 词：神经氨酸酶 前列腺肿瘤 PC-3M细胞 细胞凋亡 丁酸盐类 

分 类 号：R363[医药卫生—病理学]