灯盏花素对糖尿病大鼠肝、肾组织氧化应激的影响  

Effect of breviscapine on the oxidative stress in the liver and kidney in diabetic rats

作　　者：赵珉[1] 吴永贵[1] 林辉[1] 钱浩[1] 周典[1] 郝丽[1] 

机构地区：[1]安徽医科大学第一附属医院肾脏内科,安徽合肥230022

出　　处：《中国病理生理杂志》2005年第4期802-807,共6页Chinese Journal of Pathophysiology

基　　金：安徽省自然科学基金资助项目 (No .0 10 4 370 3)

摘　　要：目的:探讨灯盏花素对糖尿病大鼠肝、肾组织氧化应激的影响。方法:建立STZ诱导的糖尿病模型,随机分3组:正常对照组、模型组、灯盏花素给药组。8周后应用HE、油红O染色对肝组织、PAS染色对肾组织作病理检查。分光光度法检测肝、肾组织丙二醛(MDA)含量及抗氧化物酶活性。结果:HE染色显示模型组部分肝细胞脂肪变性,评分为1 .5 4±0 . 6 5 ,灯盏花素给药组评分为0 . 5 5±0 . 4 3,差异高度显著(P <0 . 0 1)。油红O染色模型组肝组织评分为2 .11±0 .82 ,灯盏花素给药组为0 . 75±0 . 6 6 ,差异高度显著(P <0 .0 1)。模型组大鼠肾重、肾重/体重、2 4h尿白蛋白排泄率(AER)与肾小球面积(AG)、肾小球容量(VG)及系膜区面积(AM)均明显增加,灯盏花素给药组这些改变减轻。灯盏花素给药组肝、肾组织MDA含量明显低于模型组(P <0 . 0 5 ,P <0 . 0 1)。超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH -Px)活性明显高于模型组(P <0 .0 5 ,P <0 .0 1)。结论:灯盏花素对糖尿病大鼠肝、肾组织有明显保护作用,其机制部分可能与抑制肝、肾组织氧化应激增加有关。AIM: To study the effect of breviscapine on the oxidative stress in the liver and kidney in diabetic rats. METHODS: Diabetes was induced by injection of streptozotocin (ST Z). Rats were randomly divided into three groups: control group, model group, mo del group treated with breviscapine. 8 weeks after STZ injection, liver lesion w as evaluated using HE, oil red O staining and kideny lesion using PAS staining. Malondiadehyde (MDA) levels and antioxidant activities in liver and kidney tissu e were determined by spectrophotometric method. RESULTS: Light microscopy in HE staining showed that liver fatty score was significantly lower in the breviscapine group compared with model ani mals (0.55±0.43 vs 1.54±0.65, P<0.01). In model group, the presen ce of cytoplasmic lipid deposits was confirmed by oil red O staining, and these changes were significantly lower in the breviscapine group than those in the mod el group (0.75±0.66 vs 2.11±0.82, P<0.01). In addition, increased kidney weight (KW), KW/body weight (KW/BW), 24 h albumin excretion rate (AER) a nd glomerular area (A G), glomerular volume (V G) as well as mesangial area (A M) on histological examination of the kidney significantly attenuated by treat ment with breviscapine (P<0.05, P<0.01). Levels of MDA were higher and sup eroxide diamutase (SOD), catalase (CAT) as well as glutathione peroxidase (GSH-P x) activities were significantly lower in liver and kidney tissue in model rats than those in control group. Breviscapine administration could all remit these c hanges (P<0.05). CONCLUSION: The mechanism of protective effect of breviscapine o n liver and kidney may be at least partly correlated with the suppression of inc rease in oxidative stress in diabetic rats.

关 键 词：糖尿病 大鼠 肝 肾 氧化性应激 灯盏花素 

分 类 号：R363[医药卫生—病理学]