人新基因hbrp的染色体定位及其对TPK活性的抑制作用(简报)  

CHROMOSOMAL LOCALIZATION AND INHIBITORY EFFECT ON TPK ACTIVITION OF A HUMAN NOVEL GENE hbrp

作　　者：张杰[1] 冯晨[1] 刘莹[1] 姜淼 杨嘉宁[1] 陈菲[1] 宋宇彤[1] 王格 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生化教研室,沈阳110001 [2]辽宁省计划生育科学研究院生殖医学重点开放实验室

出　　处：《实验生物学报》2005年第2期177-182,共6页Acta Biologiae Experimentalis Sinica

摘　　要：hbrp(Human BSP-Related Protein)是我们实验室最近在睾丸组织中克隆的一个人与BSP(bovine seminal plasma)蛋白相关的新基因.为了将有关该新基因信息与现有人类基因组转录图相整合,我们应用荧光原位杂交(fluorescent in situ hybridization-FISH)法进行了该基因的人染色体基因定位,结果成功地将hbrp基因定位在人19号染色体长臂1区3带上.hbrp基因是在对BSP蛋白功能的研究过程中发现并克隆的,其同源性分析发现,与其序列最相近的是BSP蛋白家族.hbrp基因编码的蛋白质氨基酸序列中含有四个纤连蛋白Ⅱ型结构域,而BSP蛋白结构中含有两个前后排列的纤连蛋白Ⅱ型结构域,表明该蛋白可能是一种与BSP蛋白相关的结合蛋白.以前的研究中,我们发现BSP不仅能抑制PKC的活性,还能抑制酪氨酸蛋白激酶(TPK)的活性,根据BSP的这一功能,对HBRP蛋白与TPK的关系进行了研究,结果证明HBRP蛋白对TPK活性有明显的抑制作用.现将实验方法及所观察结果报道如下:The purpose of present study is to determine the location of the novel gene hbrp which is related to bovine seminal plasma (BSP) proteins in the human chromosome by the FISH (fluorescent in situ hybridization) technique, and to investigate the relation of HBRP to TPK( ty- rosine protein kinase)by genetic engineering. The result is that the novel gene hbrp was success- fully localized on human chromosome 19q1.3; HBRP protein obviously inhibited the activity of PKC. It is concluded that the structure of BSP contained two tandemly arranged fibronectin type Ⅱ(Fn2)-domains which were related to the function of binding protein BSP. Amino acid sequence of HBRP protein with four tandemly arranged Fn2-domains showed that the protein might be binding protein functionly relating to BSP protein. We have concluded that HBRP protein obvious- ly inhibited the activity of TPK. The location of the novel gene on human chromosome 19q1.3 is very important to study the other biological functions of the protein encoded by gene hbrp.

关 键 词：HBRP 新基因 染色体定位 抑制作用 TPK 活性 荧光原位杂交 19号染色体 基因定位 人类基因组 同源性分析 睾丸组织 基因信息 人染色体 研究过程 BSP 实验室 克隆 蛋白 转录 

分 类 号：R346[医药卫生—基础医学]