氮、磷、硫对盐生杜氏藻色素积累的影响  

The Effect of Nitrogen Phosphorus and Sulfur on the Pigments Accumulation of Dunaliella salina

作　　者：孙辉[1] 徐文华[1] 雷高鹏[1] 邓婷婷[1] 杨旺贵[1] 刘玉坤[1] 曹毅[1] 白林含[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2005年第2期403-407,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技厅应用基础项目(04JY029 080 2)

摘　　要：研究了盐生杜氏藻(Dunaliellasalina)在不同浓度氮、磷、硫等营养条件下对细胞积累β胡萝卜素和叶绿素的影响.发现盐生杜氏藻细胞经过10d生长,在营养缺乏条件下其β胡萝卜素和叶绿素的比率达到最大,积累β胡萝卜素的最适条件是无氮、无磷和无硫,但是不利于盐生杜氏藻的生长.讨论了此结果的机理和意义,为利用盐生杜氏藻大规模生产β胡萝卜素提供了理论依据.Dunaliella salina is an unicellular green alga, that accumulates large amounts of β-carotene when cultivated in special stress conditions. The optimum parameters of N -NaNO3, P-KH2PO4 and S-MgSO4 for both algal important events, cell growth and accumulation of pigments, were established basis on series of experiment with different concentration under the normal conditions. Results showed that the algal cellular maximum content of β-carotene was obtained at N, P,S-free in period of 10 days. The β-carotene /chlorophyll ratio arrived the largest value, but its growth rate is limited under these condition. Based on the above results, the controlled mechanism and its effect on nitrogen, phosphate and sulfur for available cell accumulation of β-carotene was thus discussed.

关 键 词：盐生杜氏藻 氮、磷、硫 色素积累 Β-胡萝卜素 

分 类 号：Q945[生物学—植物学]