过氧化氢酶对SW480细胞应激反应的影响  

Effects of catalase on stress reaction of SW480 cells

作　　者：王群英[1] 王继德[1] 钟世顺[1] 李良仁[1] 赖卓胜[1] 张振书[1] 张亚历[1] 

机构地区：[1]南方医科大学南方医院消化科,广东广州510515

出　　处：《第四军医大学学报》2005年第8期679-681,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(01020058)

摘　　要：目的: 观察过氧化氢酶(catalase,CAT),对脂多糖(liposaccharide,LPS)诱导肠上皮SW480细胞凋亡,细胞内TNF α、IL 1β、IL 8的表达和NF κB激活的影响.方法: LPS刺激SW 480细胞后,应用流式细胞术观察细胞凋亡;采用逆转录聚合酶链反应(RT PCR)技术,检测细胞内TNF α、IL 1β、IL 8mRNA的表达水平;应用免疫电泳迁移率改变分析(elec trophoreticmobilityshiftassay,EMSA)法检测细胞内NF κB的激活.结果: CAT预孵后细胞凋亡(24 97±2 35)%较正常对照组(1 23±0 25 )%明显减少(P< 0 05 ). TNF α( 0 65±0 59)、IL 1β(0 32±0 06)、IL 8 (0 62±0 25)的表达较LPS组[ (1 33±0 94), (0 83±0 05), (1 03±0 20) ]和CAT治疗组[ (1 31±0 07 ), ( 0 82±0 04 ), ( 0 98±0 23 ) ]明显降低(P<0 05).NF κB的核结合活性也减弱,但较正常对照组仍明显增强.结论: CAT能抑制NF κB的核结合活性,降低SW480细胞对应激的反应,减少细胞凋亡.AIM: To investigate the effects of catalase (CAT) on the expression of cytokines and the activation of nuclear factor kB in intestinal epithelial cells SW480.METHODS: SW480 cells were stimulated by LPS (Liposaccharide).The apoptosis of cells was detected by flow cytometry.The expressions of TNF- α,IL-1β and IL-8 in the cells were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).The activation of NF-κB in the cells was evaluated by electrophoretic mobility shift assay (EMSA).RESULTS: Compared with the cells treated by LPS(39.67±6.12)% alone,the apoptosis(24.97±2.35)% decreased in the cells pre-treated with CAT.Furthermore,the expressions of TNF-α (0.65±0.59), IL-1β (0.32±0.06)and IL-8 (0.62±0.25) were significantly decreased ( P<0.05 ).The activation of NF-κB was also suppressed.CONCLUSION: CAT can reduce the reaction of stress SW480 cells,due to the suppression of NF-κB activity .

关 键 词：过氧化氢酶 SW480细胞 细胞凋亡 细胞因子类 NF—kB 

分 类 号：R576[医药卫生—消化系统]