一株芽孢杆菌产生的碱性纤维素酶的研究  

Studies on Alkaline Cellulase Produced by Bacillus sp. No.1:

作　　者：陈兵[1] 林开江[1] 

机构地区：[1]浙江省农科院微生物所

出　　处：《浙江农业学报》1994年第1期37-40,共4页Acta Agriculturae Zhejiangensis

摘　　要：从造纸厂废水排放口纤维垃圾中，分离出一株产生碱性纤维素酶（ＣＭＣａｓｅ）的芽孢杆菌（Ｂａｃｉｌｕｓｓｐ．）。该菌在碳氮比１∶３、起始ｐＨ５．５的条件下产酶量较高。ＣＭＣａｓｅ在ｐＨ３～１０．６时均有较高活力，以ｐＨ４．５～１０时活力稳定。Ｃａ＋＋、Ｍｇ＋＋等金属离子、ＥＤＴＡ、碱性蛋白酶和洗衣粉等均不影响其活力，最适作用温度为５５℃，在６０℃下处理１小时，酶活丧失８０％。A Bacillus strain No. 1 that produced alkaline cellulase has been isolated from waste fibre in soil near the open of drainage pipeline in the paper making factory in Hangzhou. Its higher production of the alkaline carboxymethyl cellulase (CMC ase ) required a 1∶3 ratio of carbon source to nitrogen, also an initial pH value of 5.5. The CMCase was very active over the broad pH of 3 to 10.6 and was stable in the pH of 4.5 to 10. The CMC ase activity was not affected by Ca ++ , Mg ++ , EDTA, alkaline proteinase and laundry detergents. The CMC ase had an optimum temperature of 55℃, but it was not stable in the temperature more than 60℃. It lost 80% of its activity when exposed at 60℃ for 1 h.

关 键 词：纤维素酶 芽孢杆菌 碱性 

分 类 号：Q939.97[生物学—微生物学]