Fas、bcl-2在金黄地鼠颊囊癌变过程中的表达  

The expression of Fas and bcl-2 in hamster buccal carcinogenesis

作　　者：王霞[1] 孙善珍[1] 张晓英[2] 马伯龙[1] 

机构地区：[1]山东大学口腔医院病理科,山东济南250012 [2]山东大学齐鲁医院,山东济南250012

出　　处：《上海口腔医学》2005年第2期155-158,共4页Shanghai Journal of Stomatology

基　　金：山东省科技厅科研基金(2002BB1CAA1)

摘　　要：目的:研究凋亡相关蛋白Fas、bcl-2在口腔黏膜癌变过程中的表达规律。方法:建立金黄地鼠颊囊癌变模型,采用免疫组化法检测地鼠颊囊癌变过程中凋亡相关蛋白Fas、bcl-2的表达。采用非参数检验进行统计学分析。结果:在阴性对照组黏膜,除基底细胞外,Fas多呈极强阳性表达;随上皮异常增生程度加重,Fas表达下调,主要定位于细胞的胞膜和胞质。癌变后Fas表达更低,且表达部位主要位于癌细胞胞质内,与阴性对照组黏膜相比,差异有显著性(χ2=45.576,P<0.05)。bcl-2在阴性对照组黏膜多不表达,随上皮异常增生程度加重表达上调,重度异常增生时达最高峰,鳞癌组表达降低,与异常增生组显著差异,而与阴性对照组相比,差异有显著性(χ2=19.433,P<0.05)。结论:Fas通过表达的下调及表达部位的改变,参与口腔黏膜异常增殖细胞逃逸机体的免疫监视功能及细胞毒性淋巴细胞的攻击;bcl-2通过表达上调,发挥其抑制凋亡的功能,使细胞凋亡减少,细胞的存活期延长;两者可能具有协同作用,导致增殖细胞凋亡减少,细胞增殖与凋亡失衡,导致地鼠颊囊癌的形成。PURPOSE: To study the expression of Fas and bcl-2 in oral carcinogenesis. METHODS: Using immunohistochemical method,we studied the expression of Fas and bcl-2 in DMBA induced hamster baccal carcinogenesis including negative control(14),hyperkeratosis(7),epithelial dysplasia(mild 12,moderate 7,severe 9),oral squamous cell carcinoma(OSCC)(25). Non-parametric test was used for statistical analysis. RESULTS: Fas was expressed in negative control consistently except stratum basale,and there was down-regulation in oral dysplasia and OSCC(χ2=45.576,P<0.05).bcl-2 was rarely expressed in negative control,and there was up-regulation in oral dysplasia and OSCC(χ2=19.433,P<0.05). CONCLUSIONS: Fas and bcl-2 may play an important role in inhibiting apoptosis coordinately, resulting in the imbalance of cell proliferation and apoptosis,which may contribute to oral carcinogenesis. Supported by Research Grant from Buraeu of Science and Technology of Shandong Province(No.2002BB1CAA1).

关 键 词：地鼠 癌变 FAS BCL-2 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]