氯化锂-匹罗卡品致大鼠脑髓鞘转录因子的表达及其意义  

Expression and its significance of MyT1 in the brain of epileptic rats induced by lithium chloride -pilocarpine

作　　者：李华杰[1] 包仕尧[1] 徐元[1] 张志琳[1] 吴莹[1] 

机构地区：[1]苏州大学附属第二医院神经内科

出　　处：《临床神经病学杂志》2005年第2期130-132,共3页Journal of Clinical Neurology

摘　　要：目的　探讨氯化锂匹罗卡品致大鼠脑髓鞘转录因子1 (MyT1)的表达及其意义。方法　给SD大鼠先后腹腔注射氯化锂、匹罗卡品,制成癫疒间动物模型;用免疫荧光组化法检测癫疒间大鼠癫疒间发作后不同时间大脑皮质和海马CA1 区MyT1阳性细胞数。结果　与对照组相比,癫疒间后1d组大鼠海马CA1 区MyT1阳性细胞数显著减少(P<0 .05),癫后其他各时间组大鼠脑皮质和海马CA1 区MyT1阳性细胞数均有明显的增加,其中癫疒间后7d组MyT1阳性细胞数最多(P<0. 01,P<0 .05)。结论　氯化锂匹罗卡品致疒间大鼠早期大脑MyT1表达增加,并有时程性变化。Objective To explore the expression of myelin transcription factor 1 (MyT1) and its significance in the brain of epileptic rats induced by lithium chloride-pilocarpine. Methods Models of epilepsy of SD rats were established by intraperitoneal injection with lithium chloride and pilocarpine. MyT1-positive cells in the cortex and hippocampal field CA 1 of epileptic rats were determined by immunofluorescence histochemistry.Results Compared with the control group, the number of MyT1-positive cells within the cortex and hippocampal field CA 1 of epileptic rats decreased significantly at 1 day after seizure ( P<0.05). The MyT1-positive cells significantly increased at other time points, and peaked at 7 days after seizure ( P<0.01, P<0.05). Conclusion The expression of MyT1 increases in the brain of epileptic rats induced by lithium chloride-pilocarpine at early phase and alters with time.

关 键 词：癫痢 髓鞘转录因子1 氯化锂 匹罗卡品 

分 类 号：R742.1[医药卫生—神经病学与精神病学]