阿霉素肾病肾小球硬化动物模型的研究  

The Study on Adriamycin-induced Glomerulosclerosis Rats Model

作　　者：张丽芬[1] 黄文政[2] 朱小棣[3] 王耀光[2] 

机构地区：[1]北京中医药大学东直门医院肾病内分泌科,北京100700 [2]天津中医学院第一附属医院肾内科,天津300193 [3]天津中医学院第一附属医院病理科,天津300193

出　　处：《中国中西医结合肾病杂志》2005年第4期195-199,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：天津市自然科学基金资助项目 (No .0 2 3 6115 11)

摘　　要：目的:探讨肾小球硬化不同病理发展阶段动物模型的制作。方法:采用单侧肾切除并高低剂量阿霉素尾静脉注射法建立大鼠肾小球硬化模型,观察各组大鼠血尿生化和肾组织病理改变。结果:8周时高低剂量阿霉素模型组均出现尿蛋白排出增加,血脂升高,肾功能下降;高剂量阿霉素注射组光镜下肾小球硬化达80 %以上,呈弥漫性,其中2 5 %～5 0 %呈球性硬化,肾小球脏层上皮细胞足突广泛融合或消失;肾小管病变严重,肾小管上皮细胞肿胀、颗粒变性、坏死甚至脱落入小管腔,可见大量蛋白管型;间质纤维化和大量炎细胞浸润;低剂量阿霉素注射组肾小球硬化呈局灶节段性分布,肾小球肥大,肾小囊扩张,约5 0 %肾小球有不同程度的硬化,球性硬化达10 %左右,间质可见明显纤维化和局灶性炎细胞。结论:阿霉素对肾小球脏层上皮细胞、毛细血管内皮细胞及肾小管上皮细胞均有明显毒性损伤;高低剂量阿霉素注射组均为病变稳定的肾小球硬化模型,其中高剂量阿霉素注射组肾小球硬化模型为中晚期病理改变;低剂量阿霉素注射组为肾小球硬化早期病理改变。Objective:To study glomerulosclerosis rats modeling in different development pathologic stages.Methods:Glomerulosclerosis rats model were induce by unilateral nephrectomy and injecting different doses adriamycin,and observed the blood and urine biochemistry examinations and renal pathologic changes.Results:24 hour urine protein excretion,blood lipid,blood urea nitrogen and serum creatinine in the experimental rats increased at the end of 8weeks;Lightscope and electronlicroscopy showed the fragment segmental glomerulosclerosis of model rats induced by bigger doses adriamycin injection was distributing diffusely above 80 percent, about 25%～50%glomeruli showed mostly scleroses. Glomerulus viscera epithelia cells were effacement of foot processes; tubule epithelia cells were dilatation or atrophy, lots of albumen tubule type, interstitialfibrosis, lymphocyte and monocyte soakage;Lower doses adriamycin injection rats show localized glomerulosclerosis, obesity glomerulus,and overspread bowman's scapsule,about 50 percent glomeruli suffering different degree sclerosis, 10 percent were mostly scleroses, interstitalfibrosis and localized ymphocyte and monocyte soakage.Conclusion:Adriamycin made obviously damnification to glomerulus's visceral epithelial cells, endothelial cells and tubule epithelial cells;Both two glomerulosclerosis rats model were successful made; while the bigger doses injection rats expressed as metaphase or terminal pathology glomerulosclerosis changes,the lower doses expressed as forepart glomerulosclerosis changes.

关 键 词：阿霉素肾病 肾小球硬化 动物模型 组织病理 

分 类 号：R-332[医药卫生]