松杉灵芝发酵菌丝体中水溶多糖的分离纯化与结构的比较研究  

ISOLATION, PURIFICATION AND STRUCTURAL COMPARISON INVESTIGATION OF WATER SOLUBLE POLYSACCHARIDE GFb OF FERMENTED MYCELIA FROM GANODERMA TSUGAE

作　　者：梁忠岩[1] 张翼伸[1] 苗春艳[1] 

机构地区：[1]东北师范大学生物系,长春130024

出　　处：《真菌学报》1994年第3期211-214,共4页

基　　金：国家自然科学基金

摘　　要：松杉灵芝发酵菌丝体经热水提取,冻融分级及乙醇二次分级,分离纯化出GFb级份,电泳及凝胶柱层析示其为均一多糖,分子量为9.8万。小于子实体多糖相应级份。 GFb经红外光谱,气相色谱,气质联机,碳13核磁共振,高碘酸盐氧化,Smith降解,甲基化及部分酸水解分析,确定其基本结构中主链为1→6葡萄糖基和1→6半乳糖基构戍,二者之比为1∶1,分支点在0-3位上,分枝点率为50％,与子实体多糖GF_3相同,侧链由1→3葡萄糖基,1→4葡萄糖基,末端葡萄糖基及末端半乳糖基构成,分子中分枝率为55.6％,较子实体多糖GF_3分枝率略低,分枝链略短。The water soluble polysaccharide GFb was isolated and purified from fermented mycelia of Ganoderma isugae. Its homogeneity was comfirmed by chroma-tography on Sepharose CL-4B and glass fiber electrophoresis. Its molecular weight estimated by gel filtration is 98000. By means of IR, GC, GC-MS, 13C-NMR, periodate oxidation, Smith degradation,, methylation analysis and partial hydrolysis, etc., the structure of GFb was identified. Its basic structure is composed of (1-6)-linked galactose and (1-6)-linked glucose residues in main chain, and (1-)-linked galactose, (1-3)-linked and (1-4)-linked glucose residues in side chains. Side chains attached to 0-3 of glucose and galactose residues in main chain. The rate of branch points is 50 percent in main chain and branch rate is 55.6 percent in molecules.

关 键 词：松杉灵芝 发酵 菌丝体 多糖 

分 类 号：Q939.506[生物学—微生物学]