川崎病患儿血清MMP-9和TIMP-1的水平测定  

Measurement of serum MMP-9 and TIMP-1 levels in patients with Kawasaki disease

作　　者：洪华[1] 邓君[1] 彭茜[1] 梁敏[1] 张玲英[1] 

机构地区：[1]四川省人民医院检验科,成都610072

出　　处：《江西医学检验》2005年第2期107-108,165,共3页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的了解血清MMP-9和TIMP-1在川崎病(KD)患儿中的水平及临床意义。方法采用ELISA双抗夹心法测定血清MMP-9和TIMP-1水平;采用终点散射比浊法,利用德灵BNProSpec特种蛋白分析仪检测血清超敏CRP(hs-CRP)水平。结果30例KD患儿大剂量丙种球蛋白静脉滴注前(静丙前)MMP-9水平与静脉滴注后(静丙后)和对照组比较均有显著性差异(P<0.01),静丙后与对照组差别无意义;静丙前和静丙后血清TIMP-1水平和对照组比较均有显著差异(P<0.01),但是静丙前和静丙后差别无意义;KD患儿血清MMP-9和TIMP-1水平与hs-CRP水平无相关性。结论血清MMP-9和TIMP-1参与了KD血管炎的发病机制。Objective To study the changes of serum MMP-9 and TIMP-1 levels in patients with Kawasaki Disease(KD) and evaluate their clinical significance. Methods Serum MMP-9 and TIMP-1 were detected by sandwich ELISA. Serum hs-CRP level was measured by terminal latex agglutination nephelometry immunoassay with BEHRING BN ProSpec. Results The serum level of MMP-9 in KD before high-dose intravenous gama globulin(pre-IVIg) was significantly higher than those of post-IVIg and healthy controls(P<0.01). The serum levels of TIMP-1 in KD with pre-IVIg and post-IVIg were significantly higher than that of in healthy controls(P<0.01). There was no positive correlation between MMP-9, TIMP-1 and hs-CRP in KD. Conclusion MMP-9 and TIMP-1 may be involved in the pathogenesis of KD vasculitis.

关 键 词：血清MMP-9 TIMP-1 川崎病 患儿 水平测定 ELISA双抗夹心法 大剂量丙种球蛋白 静脉滴注 散射比浊法 分析仪检测 显著性差异 对照组 临床意义 特种蛋白 发病机制 KD 相关性 P水平 血管炎 差别 

分 类 号：R734.2[医药卫生—肿瘤] R575.2[医药卫生—临床医学]