CK-19检测在乳腺癌前哨淋巴结微转移诊断中的价值  

The study of micrometastases in sentinel lymph nodes of breast cancer

作　　者：付荣湛[1] 阮长乐[1] 于学智[1] 曹莉莉[1] 孙青[1] 顾禾[1] 张丽丽[1] 

机构地区：[1]山东省千佛山医院,山东济南250014

出　　处：《山东医药》2005年第8期9-11,共3页Shandong Medical Journal

基　　金：山东省卫生厅科研基金资助项目 (2 0 0 1CA1DCA2 )

摘　　要：目的　探讨角蛋白- 19(Keratin- 19,CK- 19)检测在乳腺癌前哨淋巴结(SL N)微转移诊断中的价值。方法　采用亚甲蓝生物染色的方法对4 4例 、期乳腺癌患者的SL N进行活检,均行冰冻病理切片、石蜡切片(SE染色)检查;采用逆转录聚合酶链反应(RT- PCR)技术进行CK- 19检测。结果　SL N冰冻病理切片、石蜡切片示癌转移率分别为15 .91% (7/ 4 4 )、18.18% (8/ 4 4 ) ;CK- 19检测的阳性表达率为34.0 9% (15 / 4 4 )。其中,冰冻和石蜡切片阳性的SL N中CK- 19均阳性表达。CK- 19在SL N中的阳性表达率明显高于其冰冻及石蜡病理切片阳性率(P<0 .0 5 )。结论　CK- 19检测能提高乳腺癌SL N微转移的检出率,降低其假阴性率。Objective To evaluate the value of keratin-19mRNA examination in micrometastases of sentinel lymph nodes(SLN) of breast carcinoma.Methods In 60 breast cancer patients,methylene blue was used to locate the SLNs.SLN was successfully identified in 44 of the 60 cases.Beside the routine pathological examination,expression of CK-19mRNA was detected using reverse transcripase-polymerase chain reaction(RT-PCR) in every SLN in 44 cases whose SLN was successfully identified.Results In 8 of the 44 cases,SLN metastases was detected by both routine pathological HE staining and CK-19 RT-PCR.The expression of CK-19 was positive in 7 of the remaining 36 SLN.Conclusions The CK-19 RT-PCR method is more sensitive than routine pathological method for detection of micrometastases in SLN.This new method is better in differentiating breast cancer patients at risk for micrometastases than those with negative SLN tested by routine pathological method.

关 键 词：乳腺癌 前哨淋巴结 微转移 角蛋白-19 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]