益骨胶囊对去卵巢骨质疏松大鼠骨组织TGF-β_1mRNA表达的影响  

Effects of Benefiting-bone Capsule on the Expression of Transforming Growth Factor-β_1 mRNA in Bone Tissue of the Ovariectomized Rats

作　　者：张荣华[1] 杨丽[1] 朱晓峰[1] 蔡宇[2] 黄丰[2] 

机构地区：[1]暨南大学附属第一医院,广州510632 [2]暨南大学药学院,广州510632

出　　处：《中药材》2005年第4期312-315,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金 (No 30472274);广东省自然科学基金重点项目(No 04105837);国家中医药管理局中医药科学研究专项(No 04 05JL19)

摘　　要：目的:观察益骨胶囊预防和治疗用药对去卵巢骨质疏松大鼠骨组织TGF- β1mRNA基因表达的影响,在分子水平上阐明益骨胶囊防治骨质疏松症的可能作用机制。方法:从72只10月龄SD雌性大鼠中随机抽取24只为假手术A和B组,余48只待手术造模后随机分为4组:模型A组、模型B组、预防组、治疗组,每组12只;以双侧卵巢切除法复制骨质疏松模型;预防各组均在手术后3天即开始灌胃,假A组、模A组灌胃NS3ml、预防组灌胃益骨胶囊水溶液3ml,每天1次,共24周;治疗各组均在手术后第13周即开始灌胃,假B组、模B组灌胃NS3ml、治疗组灌胃益骨胶囊水溶液3ml,每天1次,共12周。FQ- PCR方法检测骨组织TGF- β1mRNA基因表达。结果:大鼠造模24周时,益骨胶囊预防组和治疗组骨组织TGF- β1mRNA基因表达均明显高于模型组,与假手术组相近。结论:益骨胶囊能促进骨组织TGF- βmRNA基因表达。Objective: To investigate the effects of Benefiting-bone capsule (BBC) on the expression of transforming growth factor-β_1 mRNA in bone tissue of the ovariectomized rats to further explain the mechanism of BBC on preventing and treating osteopoprosis. Methods: Twenty- four rats were randomly chosen from 72 female SD rats aged 10 months, which were classified into group A and B of sham operation dealing with normal physiological saline. The left 48 rats were randomly divided into 4 groups after ovariectomy: group A and B of osteoporotic animal model with normal physiological saline, prevented and treated groups of osteoporotic animal model with BBC. Each group included 12 rats. Ovariectomy was used to establish the osteoporotic animal model and the expression of TGF-β_1mRNA on gene level in bone tissue was detected by real time fluorescence quantitative PCR(FQ-PCR) method. Results: The expression of TGF-β_1mRNA on gene level in bone tissue of the groups of osteoporotic animal model with BBC were all higher than that of the groups of osteoporotic animal model, which were close to the groups of sham operation. Conclusion: BBC can improve the expression of TGF-β_1mRNA on gene level in bone tissue, which may be the mechanism of BBC on preventing and treating osteoporosis.

关 键 词：TGF-β1 益骨胶囊 大鼠骨组织 mRNA表达 去卵巢 防治骨质疏松症 Q-PCR方法 SD雌性大鼠 骨质疏松模型 基因表达 预防和治疗 治疗组 作用机制 分子水平 手术造模 双侧卵巢 手术后 水溶液 灌胃 B组 切除法 12周 随机 

分 类 号：R575.2[医药卫生—消化系统] R285.5[医药卫生—内科学]