胰腺癌细胞Flt-1受体表达与p53、ras基因突变的关系  

The relationship between the expression of vascular endothelial growth receptor 1(Flt-1) and p53, ras gene mutation in pancreatic carcinoma cells

作　　者：方汝亮[1] 秦仁义[2] 刘志梅[1] 颜廷俊[1] 盖学银[1] 王欣[2] 

机构地区：[1]山东省济南市第三人民医院普外科,250101 [2]华中科技大学同济医院普外科

出　　处：《临床外科杂志》2005年第4期219-220,共2页Journal of Clinical Surgery

基　　金：国家自然科学基金资助项目 (项目编号 :30 2 71 4 73)

摘　　要：目的　探讨胰腺癌细胞Flt- 1受体表达与p5 3、ras基因突变之间的关系,了解Flt- 1受体表达与p5 3、ras基因突变在胰腺肿瘤血管形成中的作用。方法　采用免疫组织化学EnVisionTM方法检测40例原发性胰腺癌组织Flt- 1受体和p5 3、ras基因的表达;采用χ2 检验分析Flt- 1受体表达与p5 3、ras突变基因的相关性。结果　Flt- 1受体和p5 3、ras基因的阳性表达率分别为77.5 %(3 1/4 0 )和5 0 % (2 0 /4 0 )、60 % (2 4/4 0 )。经连续切片对比分析表明,在Flt 1受体表达较高的区域,p5 3、ras基因的表达亦较强,Flt 1受体表达与p5 3基因突变呈显著相关性(χ2 =4.3 5 ,P <0 .0 5 ) ,但与ras基因表达之间无显著相关性(χ2 =2 .12 ,P >0 .0 5 )。结论　胰腺癌细胞Flt 1受体表达与p5 3基因突变之间有显著相关性,与ras基因突变之间无显著相关性,提示野生型p5 3基因发生突变可能会失去抑制Flt 1受体表达的功能,从而导致胰腺肿瘤血管的新生,促进肿瘤细胞生长;ras突变基因可能通过其他的途径参与胰腺肿瘤新生血管的形成。Objective To investigate the relationship between the expression of vascular endothelial growth factor receptor 1(Flt-1) and p53 , ras gene mutation in pancreatic carcinoma cells, the aim of which was to explore the effect of Flt-1 receptor expression and p53 ras gene mutation on the angiogenesis of pancreatic neoplasms. Methods The expressions of P53 gene, ras gene and Flt-1 receptor were detected by EnVisionTM method in 40 patients with primary pancreatic carcinoma tissues, and we analysed the mutual relationship between the expression of Flt-1 receptor and P53 , ras gene mutation by statistical method-χ 2 test.Results The positive expression rates of Flt-1 receptor, p53 gene and ras gene were 77.5%(31/40), 50%(20/40) and 60%(24/40) respectively. Comparative Study of the serial sections revealed that in the areas with higher Flt-1 receptor expression there were more intensive P53 gene and ras gene expression. But χ 2 test showed that Flt-1 receptor expression was only correlated with p53 gene (χ 2=4.35,P<0.05), but not with ras gene (χ 2=2.12,P>0.05). Conclusion Flt-1 receptor expression was correlated significantly with p53 gene mutation, but not with ras gene mutation in pancreatic carcinoma cells. The findings suggested that it may result in the angiogenesis of pancreatic neoplasms and promote the growth of tumor cells after the wild type p53 gene mutated; ras mutation may take part in the angiogenesis of pancreatic neoplasms through other approaches.

关 键 词：Fit-1受体 p53突变基因 RAS基因 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]