小麦重组自交系群体HMW-GS的检测与分析  

Identification of High Molecular Weight Glutenin Subunits (HMW-GS) from the Population of Recombinant Inbred Lines (RIL) of wheat

作　　者：孙海艳 李斯深[2] 崔成健 潘累斌 王绍红 

机构地区：[1]青岛市种子站,山东青岛266071 [2]山东农业大学农学院,泰安271018

出　　处：《种子》2005年第4期23-25,共3页Seed

基　　金：山东省科技厅资助项目。

摘　　要：利用重组自交系群体——RIL 8群体的131个系为材料,检测和分析了其高分子量麦谷蛋白亚基及亚基组合。结果表明,RIL 8群体Glu A1、Glu B1、Glu D1位点编码的亚基分别为1、N, 7+9、7+8和5+10、2+12,主要存在7种亚基组合类型。不同亚基及亚基组合类型在相同位点上仅存在1对等位基因差异,可以用其进行相同位点不同亚基及亚基组合对品质性状效应值的估算。High molecular weight glutenin subunits (HMW-GS) from a population of recombinant inbred lines (RIL) of wheat were tested and analyzed, which included 131 lines obtained from a cross between Chuan 35 050 and Shannong 483 in this paper. The results showed that the HMW-GS of RIL-8 at Glu-A 1, Glu-B 1 and Glu-D 1 loci were 1, N, 7+8, 7+9 and 5+10, 2+12, respectively. There were seven main HMW subunit combinations types in different lines of RIL-8.The different subunits at the same locus were different only from one Glu-1 locus, so were the different subunit combinations. Thus, the different subunits at the same loci and different subunit combinations at different loci could be esfimafed their effects value on wheat quality traits.

关 键 词：重组自交系群体 检测 高分子量麦谷蛋白亚基 HMW GS 小麦 亚基组合 组合类型 基因差异 品质性状 位点 效应值 

分 类 号：S651[农业科学—果树学] S512.1[农业科学—园艺学]