彗星实验检测吸毒人群淋巴细胞DNA  

Monitoring DNA damage in human lymphocyte cells of drug-users with comet assay

作　　者：王凤婕[1] 林健燕 罗兰[1] 吴磊[1] 谭晓东[1] 

机构地区：[1]武汉大学公共卫生学院卫生学教研室,430071

出　　处：《中国公共卫生》2005年第1期35-36,共2页Chinese Journal of Public Health

基　　金：比利时政府对外援助与协作组织资助(VVOB)

摘　　要：目的应用彗星实验方法对吸毒人群淋巴细胞DNA损伤情况进行检测。方法在湖北省某市戒毒所随机抽取123名吸毒人员作为暴露组,同时选择79名非吸毒者作为对照组,进行彗星实验。结果暴露组和对照组的彗星细胞拖尾率分别为10.03%和1.26%,差异有统计学意义;两组的彗星细胞拖尾的尾长分别为(2.35±0.88)和(2.05±0.76)μm,差异有统计学意义;吸毒年限长者彗星细胞拖尾率(11.49%)显著高于吸毒年限短者(2.98%)。多因素非条件Logistic回归分析结果表明,吸毒人群DNA损伤的可能性明显高于对照组。结论吸毒对人体淋巴细胞DNA有一定损伤,吸毒时间越长,DNA损伤越严重。Objective DNA damage in human lymphocyte cells of drug-users was monitored with comet assay.Methods 123 drug-users were randomly selected as exposure group from a detoxification setting in Hubei and 79 not exposed to drug as control group.DNA damage in their lymphocyte cells was detected with comet assay.Results Rate of DNA tail was 10.03% in exposure group,significantly higher than that in control group (1.26%),with very statistical significance,and tail length was (2.35±0.88)μm in exposure,significantly higher than that in control group (2.05±0.76) um.Rate of DNA tail was 11.49% in exposure group with long length of drug-addicts,significantly higher than that with short length of drug-addicts (2.98%).Multivariate unconditional logistic regression analysis showed that possibility of DNA damage was significantly higher in exposure group than that in control group (P<0.05).Conclusion Drug-addicts could cause certain damage to human lymphocyte cell DNA,the longer time of drug-addicts,the more serious damage of DNA.

关 键 词：彗星实验 吸毒人群 淋巴细胞 DNA损伤 

分 类 号：R394.6[医药卫生—医学遗传学]