饮服从业人员沙门菌耐药性及质粒特征分析  

Analysis on determination of resistance to antibiotics and plasmid profiles for 186 Salmonella strains from staffs of service trades of food and beverage

作　　者：李建栋[1] 陈义忠[2] 楚心唯[1] 吴敏[1] 吴爱军[1] 陈清[1] 

机构地区：[1]第一军医大学流行病学教研室,广州510515 [2]广东省广州市东山区疾病预防控制中心

出　　处：《中国公共卫生》2005年第2期205-206,共2页Chinese Journal of Public Health

基　　金：广州市东山区科技局基金项目 (2 0 0 0 QE 2 7)

摘　　要：目的　了解广州市服务和饮食行业人员所携带沙门菌的耐药性和质粒特征。方法　药敏试验采用改良K B法 ,质粒图谱分析参考Kado和Liu的方法。结果　该人群携带的沙门菌耐药率达 97 3%(1 81 / 1 86 ) ,对氨苄青霉素、氯霉素、卡那霉素、复方新诺明、庆大霉素、新霉素、妥布霉素、丁胺卡那霉素敏感性较高 ,敏感率达 84 4%～95 5 %,对氟哌酸无耐药菌株产生。对链霉素、痢特灵、羧苄青霉素及四环素的耐药率较高 ,在 1 2 9%～ 4 5 7%之间 ,对红霉素的耐药率高达 94 9%。耐 1种、2种和 3种抗生素的菌株分别占 30 6 %,32 8%和 1 9 9%。质粒阳性率为4 2 5 %(79/ 1 86 )。质粒携带株每株含质粒数量 1～ 6个不等 ,分子量大小为 0 2 1～ 2 6 72Md ,质粒条带数以 1～ 3条居多 ,未发现耐药谱与质粒特征有关。结论　广州市服务和饮食行业人员携带的沙门菌耐药情况比较谱遍 ;检测沙门菌质粒阳性率不高 ,质粒特征与耐药谱无关。Objective To determine resistance to antibiotics and plasmid DNA fingerprint of a total of 186 Salmonella strains isolated from staffs of service trades of food and beverage. Methods Antibiotic resistance testing was performed by the Kirby-Bauer disk diffusion method.Plasmid profiles of the strains were examined using the mehod described by Kado and Liu with some modification.Results 1) Of the bacterial isolates tested, 97.3%(181/186)showed drug-resistance.Single-drug resistance to erythomycin(EM)(94.9%)was the most frequent.The rates of single-drug resistance to tetracycline(TC),streptomycin(SM),carbenicillin(CP)and furazolidone(FUZ)accounted for 45.7%,29.0%,26.5% and 12.9% respectively.The rates of sensitivity to aminopenicillin(AMP),chloramphenicol(CMP),kanamycin(KM),complex sulfamethoxazole-trimethoprim(COS),gentamicin(GN),neomycin(NM),tobramycin(TOB),amikacin(AKN) and norfloxacin(NOR) were high,between 84.4% and 95.5%.Regarding the durg-resistance patterns,30.6% of the isolates were resistant to a single durg,while 69.4% were multi drug-resistant.Two-drug resistance to EM/TC and thrid-drug resistance to EM/TC/SM or EM/TC/CP were the second-most frequent and the three-most frequent.2)79 out of 186 Salmonella strains were found to contain at least one plasmid,with the size of the plasmids ranged between 0.21 and 26.72 Mda.1-3 of plasmid were frequently seen.No relationship was found between patterns of drug-resistance and plasmid profiles.Conclusion The drug-resistance of salmonella from carriers are common in Guangzhou.The detection rate of plasmid of the strains is low and no direct correlation presents between antibiotic resistance patterns and plasmid profiles.

关 键 词：沙门菌 质粒图谱 耐药性 

分 类 号：R155.6[医药卫生—营养与食品卫生学]