瘦素和神经肽Y mRNA与肥胖抵抗的关系  

Study on relation between leptin,NPY mRNA and obesity-resistant

作　　者：翟玲玲[1] 姚兴家[1] 王博[1] 白英龙[1] 吴辉[1] 郑琳琳[1] 刘玉芬[1] 

机构地区：[1]中国医科大学公共卫生学院儿童少年卫生学教研室,沈阳110001

出　　处：《中国公共卫生》2005年第3期259-260,共2页Chinese Journal of Public Health

摘　　要：目的　研究膳食诱导肥胖 (DIO)及膳食诱导肥胖抵抗 (DIO -R)大鼠血清瘦素水平及下丘脑弓状核神经肽YmRNA(NPYmRNA)的表达情况 ,探讨瘦素、神经肽YmRNA与肥胖抵抗的关系。方法　采用放射免疫方法检测血清瘦素水平 ,原位杂交方法检测神经肽YmRNA表达。结果　 2周末 ,DIO -R大鼠神经肽YmRNA水平显著低于DIO大鼠 (P <0 0 5 ) ,而瘦素水平高于DIO和对照组大鼠 (P <0 0 5 ) ;12周末 ,DIO -R大鼠瘦素水平低于DIO大鼠 (P <0 0 5 ) ,高于对照组大鼠 (P <0 0 5 )。结论　保持瘦素敏感性和神经肽YmRNA下降可能与肥胖抵抗有关。Objective To study the level of leptin and NPY mRNA of DIO and DIO-R rats and the relation between NPY,leptin and obesity-resistant.Methods The level of leptin were determined by radioimmunnoassay.The NPY mRNA were detected in situ hybridization.Results At week 2,the NPY mRNA expression of DIO-R rats were lower than those of DIO rats( P <0 05),the level of leptin of DIO-R rats was higher than that of DIO rats and the control( P <0 05);At week 12,the level of leptin of DIO-R rats were decreased as compared with DIO rats and increased as compared with the control( P <0 05).Conclusion Decreased NPY mRNA and the kept leptin sensitivity might be related to obesity-resistant.

关 键 词：膳食诱导肥胖 膳食诱导肥胖抵抗 神经肽Y(NPY) 瘦素 原位杂交 

分 类 号：R151.2[医药卫生—营养与食品卫生学]