应用组织芯片技术研究bcl-10、TopoⅡα及Ki-67在MALT型淋巴瘤中的表达和意义  

Significance of bcl-10,TopoⅡα and Ki-67 in mucosa-associated lymphoid tissue lymphoma:a tissue microarray study

作　　者：刘冬梅[1] 王晋芬[2] 张建中[3] 

机构地区：[1]山西医科大学汾阳学院病理教研室,汾阳032200 [2]山西省肿瘤医院病理科,太原030013 [3]解放军306医院病理科,北京00101

出　　处：《白血病．淋巴瘤》2005年第2期92-95,共4页Journal of Leukemia & Lymphoma

摘　　要：目的应用组织芯片技术研究bcl蛳10、TopoⅡα及Ki蛳67在MALT型淋巴瘤中的表达及意义。方法建立包含84例MALT型淋巴瘤、7例弥漫性大B细胞淋巴瘤(DLBCL)、17例其他肿瘤及18例淋巴结反应性增生,共252点阵的组织芯片,应用免疫组织化学技术(S蛳P法)检测bcl蛳10、TopoⅡα及Ki蛳67在MALT型淋巴瘤中的表达。结果Ki蛳67、TopoⅡα的表达在ⅠE期与Ⅱ2E+Ⅲ期之间差异有显著性(P<0.05),并且与临床分期呈正相关(r=0.423,P=0.000;r=0.299,P=0.016)。淋巴结反应性增生中生发中心B细胞胞质大量表达bcl蛳10,边缘区中等量表达,在套区B细胞较少表达;在MALT淋巴瘤中,bcl蛳10核型表达7例(8.33%),胞质型表达27例(32.14%)。bcl蛳10胞质表达在低度和高度恶性MALT型淋巴瘤中差异有显著性(P<0.05);但与临床分期无关。核型表达者与肿瘤细胞浸润有关。结论MALT型淋巴瘤中TopoⅡα与Ki蛳67的作用相似,它们可作为预后评价的指标。bcl蛳10表达广泛地存在于MALT型淋巴瘤中,该基因异常表达可能与肿瘤恶性程度有关,可作为判断预后的指标之一,核表达可能与病情的进展有关,与肿瘤侵袭行为有关。Objective To investigate the expression and significance of bcl- 10, TopoⅡαand Ki- 67 in marginal zone B cell lymphoma (MZL) mucosa- associated lymphoid tissues (MALT) type. Methods Created 252- dot tissue microarray consisting of 84 cases of MALT lymphomas, 7 cases of diffuse large B cell lymphomas (DLBCL), 17 cases of other malignant neoplasia and 18 cases of reactivited lympho nodes. A S- P method was performed to examine the expression of all markers. Results The expression of Ki- 67 and TopoⅡα was significantly different between grade ⅠE and Ⅱ2E+Ⅲ (P <0.05) and correlated obversely to clinical grade (r =0.423,P =0.000;r =0.299,P =0.016). In activited lymph follicles, cytoplasmic expression of bcl- 10 protein was abundantly in the germinal center B cell, moderately in the marginal zone, and rarely in the mantle zone B cell. bcl- 10 expressed in nuclear (8.33 %) and cytoplasimci (32.14 %) in MALT lymphoma. Cytoplasmic bcl- 10 expression in low- grade MALT lymphoma was significantly different from that in high- grade MATL lymphoma (P <0.05); but, there were no correlation to clinical stage. Nuclear bcl- 10 expression in MALT lymphoma was correlated with aggression of tumor cells (P <0.05). Conclusions Ki- 67 and TopoⅡα may be regard as the prognosistic factor. Expression of bcl- 10 is found in the most cases of MALT lymphoma, which may be correlated with the increasing malignancy and can also act as one of prognostic factors. Bcl- 10 nucleus expression may be correlated with progression of this tumor.

关 键 词：组织芯片 BCL-10 TopoⅡα KI-67 MALT淋巴瘤 

分 类 号：R733.1[医药卫生—肿瘤]