胃癌细胞周期和nm23-H1基因表达与淋巴结转移的关系  

The relationship between expression of nm23-H1 and lymphnods metastasis

作　　者：吴亮[1] 王颖[1] 蔡刚祥[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2005年第2期67-69,共3页Journal of Critical Care In Internal Medicine

摘　　要：目的:探讨胃癌组织nm23- H1 基因表达与细胞周期及其淋巴转移的关系。方法:收集手术切除正常胃黏膜及胃癌组织标本各28 例,采用流式细胞技术进行细胞周期分析及nm23 -H1蛋白半定量检测。结果:细胞周期分析显示正常胃黏膜组织均为二倍体;28例胃癌组织中有18 例为异倍体,14/18例(77.78%)异倍体瘤中有发生淋巴结转移,10 例非异倍体瘤中仅有3 例出现淋巴结转移(30%),两者比较有显著性差异(P<0.05)。正常胃粘膜组织增殖指数(PI)为2.04%,胃癌组织PI为12.69%±6.78%。其中淋巴结转移阳性组胃癌组织PI为14.86%±8.41%,显著高于淋巴结转移阴性组胃癌组织(PI为10.51%±4.89%)(P<0.05)。正常胃粘膜组织nm23 基因表达为阴性,胃癌组织nm23 基因表达(FI)为0.73±0.36,其中淋巴结转移阳性组FI 为0.42±0.24,淋巴结转移阴性FI 为1.04±0.17,两者比较有显著性差异(P< 0.05)。非异倍体组胃癌nm23基因表达(FI)为1.01±0.21,显著高于异倍体组胃癌(FI为0.45±0.29)(P<0.05)。结论:胃癌淋巴转移与胃癌组织的异倍性、高增殖活性及nm23 H1基因表达缺失有关。Objective:To investigate the relationship between expression of nm23-H1 gene and lymph node metastasis. Methods: FCM was used to detect protein expression of nm23-H1 gene and cell cycle distribution. Results:All normal gastric tissue cells were diploid,18 of 28 gastric cancer showed heteroloid, Lymph node metastasis were detected in 14 of 18( 77.78%) heteroloid tumors and in 3 of 10 (30%) nonheteroloid tumor. PI(proliferation index) was 2.04% in normal gastric tissues. And PI of gastric carcinoma was 12.69%± 6.78%. PI of gastric carcinoma with lymph node metastasis was 14.86%± 8.41% higher than gastric carcinoma without lymph node metastasis ( 10.51%± 4.89%). (P< 0.05) Expression of nm23-H1 was negative in normal gastric tissues. FI of nm23-H1 was 0.73± 0.36. FI(fluent index) of gastric carcinoma with lymph node metastasis was 0.42± 0.24, lower than gastric carcinoma without lymph node metastasis ( 1.04± 0.17). (P< 0.05).FI of nm23-H1 gene was 1.01± 0.21 in nonheteroloid gastric carcinoma higher than heteroloid gastric carcinoma ( 0.45± 0.29)(P< 0.05). Conclusion:Lymph node metastasis of gastric carcinoma is related to heteroloid、high proliferation and expression nm23-H1.

关 键 词：细胞周期 NM23-H1 胃肿瘤 

分 类 号：R573.9[医药卫生—消化系统]