X射线诱导C6胶质瘤细胞凋亡的研究  

Induction of apoptosis of C6 glioma cells by X radiation

作　　者：黄齐兵[1] 李新钢[1] 李刚[1] 王东海[1] 王新宇[1] 张元鹏[1] 

机构地区：[1]山东大学齐鲁医院神经外科,山东济南250012

出　　处：《肿瘤防治杂志》2005年第7期499-503,共5页China Journal of Cancer Prevention and Treatment

基　　金：山东省卫生厅资助项目(1998CAICJA8)

摘　　要：目的探讨X射线能否诱导胶质瘤细胞凋亡及剂量-效应关系。方法用不同剂量X射线照射细胞,并分别在不同时间段用倒置显微镜、电镜观察形态变化;DNA凝胶电泳观察阶梯状条带形成;流式细胞仪检测凋亡峰及细胞凋亡百分率。结果20Gy照射后48h观察组细胞凋亡率最高(38.49%)。结论放射可诱导胶质瘤细胞凋亡,20Gy的照射剂量对胶质瘤有最强的诱导凋亡作用,但不存在时间效应关系。OBJECTIVE:To determine whether apoptosis of glioma cells can be induced by X-ray radiation,and to appropriate the dose and acting time.METHODS:The glioma cells were irradiated by different doses at different time and they were observed by the micro-scope, electromicroscope, DNA agarose gel electrophoresis and flow cytometry, and the results were analysed.RESULTS:Cell apoptosis rate of the group(20Gy,48h) was the highest,38.49%.CONCLUSION:Radiation can induce the apoptosis of C6 glioma cells and 20 Gy radio-dose has the most powerful effect on C6 glioma cells.

关 键 词：神经胶质瘤 脑肿瘤 细胞死亡 

分 类 号：R739.4[医药卫生—肿瘤]