pcDNA3.1-asHpa对高转移性人乳腺癌细胞系侵袭力和黏附力的影响  

Inhibition of invasiveness of human mammary carcinoma cell line MDA-MB-435S by pcDNA3.1-asHpa

作　　者：阮绪芝 黄立英[2] 李瑞明 陈霞萍[1] 王卫民[1] 

机构地区：[1]郧阳医学院附属太和医院生命科学研究所,湖北十堰442000 [2]中山大学医学院达安基因公司,广东广州510089

出　　处：《肿瘤防治杂志》2005年第7期508-510,共3页China Journal of Cancer Prevention and Treatment

基　　金：湖北省教委科研发展基金资助项目[(2001)022号]

摘　　要：目的探索pcDNA3.1asHpa对高转移性人乳腺癌细胞株MDA MB435S侵袭力和黏附力的作用。方法以质粒pcDNA3.1为载体,将乙酰肝素酶信号密码的DNA片段反向插入得到反义乙酰肝素酶信号密码真核表达载体(pcDNA3.1asHpa),以脂质体共转染人乳腺癌细胞MDA MB435S,以Boyden侵袭小室实验检测癌细胞侵袭力的变化,以细胞贴壁能力的变化检测其黏附力的变化。结果与正常对照组(侵袭率为54.3%,贴壁细胞数为22.91/视野)及空载体转染组(侵袭率为42.6%,贴壁细胞数为19.17/视野)相比,经pcDNA3.1asHpa转染的细胞侵袭力(16.3%)受到一定的抑制,黏附力(贴壁细胞数为21.36/视野)没有明显的变化。结论pcD NA3.1asHpa对人乳腺癌细胞株MDA MB435S的侵袭力有一定的抑制作用,对黏附力无显著影响。OBJECTIVE:To evalute the inhibitory effect of pcDNA3.1-asHpa on the invasiveness of human mammary carcinoma cell line MDA-MB-435S.METHODS: Human heparanase signal code DNA was inserted into the expression plasmid pcDNA3.1 site in an antisense orientation to produce pcDNA3.1-asHpa, and the pcDNA3.1-asHpa was embedded in cationic liposome lipofectin and transfected into MDA-MB-435S cells. The invasiveness of transfected MDA-MB-435S was measured quantitatively by the matrigel invasion assays. The adhesion of transfected MDA-MB-435S was estimated quantitatively by measuring cells adhered to the wall during 4 h.RESULTS: The invasiveness of MDA-MB-435S cells treated with pcDNA3.1-asHpa(16.3%) significantly decreased compared with both that of the control group (54.3%) and the pcDNA3.1 transfected group (42.6%). However, the adhesiveness had no significant change. CONCLUSION: The pcDNA3.1-asHpa has a significant inhibitory effect on the invasiveness of human mammary carcinoma cell line MDA-MB-435S.

关 键 词：葡糖醛酸糖苷酶 乳腺肿瘤 肿瘤细胞 培养的 肿瘤转移 细胞黏附 

分 类 号：R737.9[医药卫生—肿瘤]