TRAIL与顺铂联用对人骨肉瘤细胞杀伤作用的实验研究  

KILLING EFFECT OF COMBINED APPLICATION OF TRAIL AND CISPLATIN ON OSTEOSARCOMA CELLS

作　　者：黄涛[1] 吕刚[1] 李建华[2] 孙洁[2] 高大新[1] 王岩峰[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学附属第一医院病理中心

出　　处：《中国组织化学与细胞化学杂志》2005年第2期191-195,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的　探讨TRAIL与顺铂联合应用对人骨肉瘤细胞的杀伤作用。方法　将TRAIL与顺铂单独及联合应用于体外培养的骨肉瘤OS 732细胞, 采用MTT法测定细胞抑制率, 于倒置相差显微镜、荧光显微镜、扫描及透射电镜下观察细胞的形态学改变及超微结构变化。结果　50ng/ml的TRAIL与5μg/ml顺铂合用于OS- 732 细胞24 小时后, 细胞抑制率为84 .37%, 明显高于单用TRAIL (50μg/ml) 时的9 .68%及单用顺铂(5μg/ml) 时的24 .39% (P<0 01)。细胞的形态与超微结构观察均显示, 合用比单用促凋亡效应更显著。结论　TRAIL与顺铂合用, 可高效杀伤人骨肉瘤细胞, 此协同作用主要是通过诱导肿瘤细胞凋亡来实现的。Objective To study the killing effect of the combined application of TRAIL and cisplatin on human osteosarcoma cells. Methods TRAIL and cisplatin were used on the osteosarcoma cells respectively and jointly and the inhibition rate was measured by MTT assay. Morphologicalchanges and celluar ultrastructure were observed with inverted phase contrast microscopy, fluorescence microscopy and electron microscopy. Results Twenty-four hours after the joint application of 50ng/mlTRAIL and 5μg/mlcisplatin to OS-732 cells, the inhibition rate was 84.37% by MTT essay, which was significantly higher than that of the respertive application of 50ng/ml TRAIL (9.68%) or 5μg/ml cisplatin (24.39%) (P<0.01). Changes in morphology and celluar ultrastructure indicated that the apoptosis-inducing effect of the joint application was much stronger than that of the respective applications. Conclusion Combination of TRAIL and cisplatin is obviously conducive to the killing effect on OS732 cells, which is mainly fulfilled by induing apoptosis.

关 键 词：TRAIL 顺铂 凋亡 

分 类 号：R738.1[医药卫生—肿瘤]