基因芯片检测肝炎肝硬化患者肝组织中HBVDNA  

Study on HBVDNA in the Liver Tissues of Patients with Posthepatitic Cirrhosis by Gene Chips

作　　者：曹新民[1] 赵伟[1] 刘伟[1] 刘全俊[1] 张林[1] 周镇先[1] 刘新珏[1] 

机构地区：[1]东南大学医学院附属南京市第二医院,江苏省南京市210003

出　　处：《中国全科医学》2005年第8期633-635,共3页Chinese General Practice

基　　金：江苏省科学技术委员会重点科研项目(BS2000028);江苏省卫生厅2002年重点科研项目

摘　　要：目的研究病毒性肝炎基因芯片的制备,用肝炎基因诊断芯片,免疫组织化学法和原位分子杂交法,检测99例乙型肝炎后肝硬化肝组织,比较各种方法的优缺点。方法将PCR扩增的HBVDNA探针用点样仪点于玻片介质上,并经过点样处理后制备成基因芯片;收集肝炎后肝硬化肝组织标本99份,分别用基因芯片、原位分子杂交法、免疫组织化学法检测HBVDNA和HBcAg。结果53例HBcAg、HBVDNA阳性组织中基因芯片检测阳性40例;22例HBcAg阳性组织中基因芯片检测阳性6例;32例HBcAg、HBVDNA阴性组织中基因芯片检测均阴性。结论肝炎基因诊断芯片可以检测肝组织中HBVDNA,准确率可达75%,假阳性率低。Objective To study the preparation of the gene chips diagnosing hepatitis virus.Hepatitis gene was used to diagnose HBVDNA and HBcAg respectively in the liver tissues of the 99 cases patients with posthepatitic cirrhosis,immunocytochemistry and site molecule hybridization technique.Then advantages and disadvantages of the diagnose methods were compared.Methods The gene chip was prepared by the HBVDNA microarrays prepared by spotting hybridization (PCR) of target genes putted on the specialty glass with the lattice apparatus.The expression of HBVDNA in the liver tissues of the 99 cases patients with posthepatitic cirrhosis were collected,and HBVDNA and HbcAg were tested by hepatitis virus B with gene chips,immunocytochemistry and site molecule hybridization technique.Results In the 53 samples with HBVDNA and HBcAg positive diagnosed with immunolytochemistry and in site molecule hybridization technique,there were 40 samples with positive tested by the gene chips.In the 23 samples with HBcAg positive and 32 samples with HBcAg and HBVDNA negative,6 cases were positive and 32 cases were negative by the gene chip.Conclusion HBVDNA in the liver tissues can be detected by the gene chips,the accuracy of diagnosing was 75% and the rate of the sham positive was lower.

关 键 词：基因芯片检测 肝硬化患者 肝炎基因诊断芯片 原位分子杂交法 免疫组织化学法 HBCAG HBVDNA阳性 肝炎后肝硬化 病毒性肝炎 硬化肝组织 DNA探针 PCR扩增 肝组织标本 假阳性率 优缺点 准确率 制备 点样 阴性 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R575.2[医药卫生—临床医学]