血小板衍生生长因子-BB和转化生长因子-β对人牙周膜细胞增殖的影响  

Effect of Platelet-Derived Growth Factor-BB and Transforming Growth Factor-β on the Proliferation of Human Periodontal Ligament Cells in Vitro

作　　者：吉建新[1] 廖伟娇[2] 邱志辉[3] 潘广祠[1] 张婷[2] 

机构地区：[1]广州医学院第一附属医院口腔科,广州510120 [2]广州医学院第一附属医院检验科,广州510120 [3]广州呼吸疾病研究所,广州510120

出　　处：《热带医学杂志》2005年第2期124-127,共4页Journal of Tropical Medicine

基　　金：广州市科技局资助项目(No.1999-J-008-01)。

摘　　要：目的探讨血小板衍生生长因子-BB(PDGF-BB)和转化生长因子-β(TGF-β)对人牙周膜(PDL)细胞增殖的影响。方法体外培养人PDL细胞,与不同浓度的PDGF-BB、TGF-β或PDGF-BB+TGF-β作用,用噻唑盐(MTT)法观察PDL细胞增殖的情况。结果PDGF-BB和TGF-β均明显促进PDL细胞增殖,在1～50ng/ml和5d范围内,呈浓度-时间依赖关系,PDGF-BB最佳效应时间为3d,最佳效应浓度为10ng/ml,其增殖比对照组增加了256.1%(P<0.001),TGF-β最佳效应时间为4d,最佳效应浓度为5ng/ml,比对照组增加了187.7%(P<0.01),在时间上TGF-β的促增殖作用晚于PDGF-BB。PDGF-BB与TGF-β联合应用时,二者有协同作用,可非常显著地促进PDL细胞增殖,其最大效应作用发生在用药后3d,此时与对照组相比增加了326.9%(P<0.001)。结论PDGF-BB、TGF-β可促进人PDL细胞的增殖,二者联合应用有协同效应。Objective To evaluate the biological effects i n vitro of platelet-derived growth f actor-BB(PDGF-BB)and /or transforming growth factor-β(TGF-β)on the proliferation in human period ontal ligament (PDL)cells.Methods PDL cells were obtained from healthy donors.Cells(6×10 5 /200μl)were cultured for 1to 5days in DMEM supplemented with 1%FBS alone(control),or containing PDGF-BB(1,5,10or 50ng /ml ),TGF-β(1,5,10or 50ng /ml )or both.Proliferation of the PDL cells was measured by MTT colorimeteric assay.Data was analyzed by ANOVA.Results PDGF-BB or TGF-βat concentration of 1to 50ng /ml show ed a significant effects.A signific ant enhanced of proliferation was seen when PDL cells were treated with 1～50ng /ml PDGF-B B or TGF-βin a dose-and time dependent manner(P<0.05or P<0.01).The most optimal concentration of PDGF-BB and TGF-βwas 10ng /ml and 5ng /ml,cultured for 3days and 4days,respectively.The effect of combination of PDGF-BB with TGF-βwas the most signification one(P<0.001).Conclusions PDGF-BB and /or TGF-βcan promote the proliferation of PDL cells.The data suggest that these growth factors may be valu able in promoting the regeneration o f periodontal connective tissue.

关 键 词：牙周膜细胞 血小板衍生生长因子-BB 转化生长因子-Β 

分 类 号：R781.4[医药卫生—口腔医学]