转甜菜碱醛脱氢酶基因水稻的获得  

Production of Transgenic Rice with Betaine Aldehyde Dehydrogenase Gene

作　　者：林秀峰[1] 刘志铭[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,吉林公主岭136100

出　　处：《吉林农业科学》2005年第2期7-9,共3页Journal of　Jilin Agricultural Sciences

基　　金：国家转基因植物研究与产业化开发专项(J99-B-001)资助

摘　　要：利用农杆菌介导法,将来源于山菠菜的甜菜碱醛脱氢酶基因(BADH)转到水稻品种丰优516成熟胚诱导的愈伤组织,所获得的G418抗性植株,分别在含有5g/L和8g/LNaCl的生根培养基上进行耐盐性筛选,然后对抗性植株进行PCR分子检测,最终获得73株耐盐的转BADH基因植株。By using Agrobacterium-mediated transformation, the callus derived from mature embryos of rice variety 'Fengyou 516' had been transformed with Betaine-aladehyde dehydrogenase from Atriplex hortensis. G418 resistant plants were obtained in this study and put in rooting culture containing 5g/L NaCl respectively. By using salt tolerance selection, resistant plants were obtained. PCR molecule testing indicated that 73 transgenic plants were salt tolerance.

关 键 词：甜菜碱醛脱氢酶基因 PCR分子检测 转BADH基因 农杆菌介导法 抗性植株 生根培养基 愈伤组织 水稻品种 G418 NaCl 成熟胚 山菠菜 耐盐 

分 类 号：S511[农业科学—作物学] S641.303.6