伪狂犬病毒闽A株基因文库的构建及物理图谱分析  

Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping

作　　者：汪铭书[1] 郭万柱[1] 娄高明[1] 费恩阁[1] 宣华[1] 

机构地区：[1]四川农业大学,中国人民解放军农牧大学

出　　处：《中国病毒学》1994年第4期347-350,共4页Virologica Sinica

摘　　要：本文报道以质粒ｐＢＲ３２２作载体，用鸟枪法克隆出了ＰＲＶ闽Ａ株除ＢａｍＨＩ－１，２外的所有酶切片段，构建了ＰＲＶ闽Ａ株基因文库，并以克隆出的ＢａｍＨＩ片段用光生物素标记作探针，应用分子杂交法确定了ＰＲＶ闽Ａ株绝大部分限制性内切酶位点的位置。he Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of Pseudorabies virus Min-A strain were determined.

关 键 词：伪狂犬病毒 基因文库 物理图谱 家畜病毒学 

分 类 号：S852.65[农业科学—基础兽医学]