穿心莲提取液减轻心肌缺血－再灌注过程中钙超负荷的机制探讨  

Study of the mechanism of andrographis paniculata nees (APN) in alleviating the Ca￣(2+)-overloading in the process of myocardial ischemic reperfusion

作　　者：郭志凌[1] 赵华同 郑信华[1] 

机构地区：[1]同济医科大学附属同济医院心内科

出　　处：《中国病理生理杂志》1994年第6期591-594,共4页Chinese Journal of Pathophysiology

摘　　要：本文采用整体犬急性心肌缺血－再灌注模型以探讨穿心莲提取液减轻钙超负荷的机制。结扎冠脉左前降支上１／３处９０ｍｉｎ后再灌注６０ｍｉｎ较持续结扎１５０ｍｉｎ，缺血中心区心肌细胞内Ｃａ２＋增加（Ｐ＜０．０５）、Ｎａ＋明显增加（Ｐ＜０．０１），心肌细胞膜Ｃａ２＋－ＡＴＰａｓｅ活性明显降低（Ｐ＜０．０１），心肌组织ＭＤＡ含量显著增高（Ｐ＜０．０１）。而在再灌注前４５ｍｉｎ静脉给予穿心莲提取液，则见其缺血中心区心肌细胞内Ｃａ２＋降低（Ｐ＜０．０５）、Ｎａ＋明显降低（Ｐ＜０．０１），心肌细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－ＡＴＰａｓｅ活性明显增加（Ｐ＜０．０１）。心肌组织ＭＤＡ含量显著降低（Ｐ＜０．０１）。说明穿心莲提取液减轻心肌缺血－再灌注过程中钙超负荷与减轻氧自由基危害、保护心肌细胞膜ＡＴＰａｓｅ活性、降低钠超负荷有关。Mechanism of APN in alleviating the Ca￣2+-overloading in dog model during the process of ischemic reperfusion was studied.In comparison with the sustained ischemic group,the parameters in reperfusion ischemic group showed:In central ischemic region of myocardial cell Ca￣2+ increased (P<0.05),Na￣+ increased markedly (P<0.01),Ca￣2+-ATPase activity dropped markedly (P<0.01),MDA increased significantly (P<0.01),whereas in the APN pretreated group,Ca￣2+ in relevant area reduced(P<0.05),Na￣+ decreased significantly(P<0.01),and the activity of Ca￣2+-ATPase and Na￣+-ATPase increased markedly(P<0.01),MDA decreased significantly(P<0.01).These data indicate that APN may improve the activity of Sarcolemma ATPase in alleviating the Ca￣2+ and Na￣+-overloading by decreasing the harmful effect of O_2 free radicals.

关 键 词：心肌缺血 再灌注损伤 穿心莲 钙 

分 类 号：R363.27[医药卫生—病理学] R285.5[医药卫生—基础医学]