树脂法提取柑橘皮中水溶性黄酮工艺研究  

STUDY ON EXTRACTION OF WATER-SOLUBLE FLAVONES IN ORANGE PEEL BY RESIN ADSORPTION METHOD

作　　者：张久春[1] 张清峰[1] 李楠[1] 姜子涛[1] 

机构地区：[1]天津商学院食品科学与工程系,300134

出　　处：《食品研究与开发》2005年第2期77-80,共4页Food Research and Development

基　　金：天津市自然科学基金(043604511);天津市高校科技发展基金(20030905)资助课题

摘　　要：为优化柑橘皮中水溶性黄酮类化合物的提取工艺,本文考察了几种树脂对水溶性黄酮的吸附性能。并以95%乙醇为洗脱剂进行了洗脱实验。结果表明:AB-8树脂对水溶性黄酮有很好的吸附性能,最大吸附量为30.2mg/g(水溶性黄酮/湿树脂);95%乙醇洗脱速度快,洗脱彻底,洗脱剂用量为2倍柱床体积(BV)。用该法提取的产品具有良好的水溶性,最大吸收波长为280nm,并且利用高效液相色谱对获得的提取物进行了分离测定。In order to optimize the extraction conditions of water-soluble flavones in orange peel, the adsorption abilities of some resins to water-soluble flavones in orange peel have been studied. Furthermore, the desorption conditions of the flavones have also been carried out using a 95% ethanol solution as a desorption reagent. The result indicates that the adsorption ability of AB-8 is the biggest among all resins and its maximum capacity is 30.2mg/g(water-soluble flavones/wet resin). The desorption of the flavones is fast and complete with 95% ethanol when the elution volume is 2 times of bed volume(BV). The flavones obtained by the proposed technique have a good solubility and dispersion in water, and maximum absorbance appears at 280 nm. In addition, the flavones were characterized by HPLC.

关 键 词：水溶性 柑橘皮 工艺研究 树脂法 95%乙醇 黄酮类化合物 AB-8树脂 最大吸收波长 高效液相色谱 吸附性能 最大吸附量 提取工艺 分离测定 洗脱剂 提取物 

分 类 号：TQ914.1[化学工程]