端粒酶逆转录酶mRNA在胃癌中的表达及其意义  

The expression of telomerase reverse transcriptase mRNA in gastric cancer and its implication

作　　者：汤立旦[1] 黄河[2] 孙洁[2] 张则伟[3] 

机构地区：[1]浙江医学高等专科学校,在职研究生310053 [2]浙江大学医学院附属第一医院骨髓移植中心,杭州310003 [3]浙江省肿瘤医院

出　　处：《浙江医学》2005年第4期243-245,共3页Zhejiang Medical Journal

摘　　要：目的研究人类端粒酶逆转录酶(hTERT)在胃癌组织中的表达及其在胃癌发生发展中的作用。方法应用实时荧光定量PCR技术检测22例胃癌组织及癌旁正常黏膜组织中hTERTmRNA的表达水平,分析其与肿瘤恶性程度的关系。结果胃癌组织hTERTmRNA表达水平明显高于癌旁正常组织(P<0.01),但不同胃癌TNM分期、组织分化程度之间的hTERTmRNA表达水平差别均无显著性意义(均P>0.05)。结论在胃癌组织中hTERTmRNA表达明显高于正常组织,可作为胃癌诊断的特异性指标之一。Objective To detect the expression levels of human telomerase reverse transcriptase (hTERT) and to explore its significance in the development of gastric carcinoma. Methods Quantitative real-time PCR was used to detect expression of hTERT mRNA in cancerous tissue and adjacent normal tissues of 22 patients with gastric cancers. Results The levels of hTERT mRNA in cancerous tissue were significantly higher that shoes in adjacent normal tissues (P<0.01). There were no significant differences between different TNM stages and different pathological grades of gastric cancer. Conclusion The hTERT expression levels increased in the gastric cancer, which indicates hTERT may be involved in carcinogenesis of gastric cancer.

关 键 词：端粒酶逆转录酶MRNA 表达及 其意义 人类端粒酶逆转录酶 mRNA表达 hTERT PCR技术检测 胃癌组织 实时荧光定量 肿瘤恶性程度 癌旁正常组织 TNM分期 特异性指标 发生发展 黏膜组织 方法应用 分化程度 胃癌诊断 水平 显著性 

分 类 号：R735.2[医药卫生—肿瘤]