金黄色葡萄球菌DNA对小鼠免疫功能的影响  

The Effects of Staphylococcus Aureus DNA On Immunocompetence of Mice

作　　者：历风元[1] 蒋智敏[2] 陈圆[1] 张晋湘[1] 张儒莲[1] 胡向东[1] 

机构地区：[1]南昌市第一医院呼吸内科,江西南昌330008 [2]泸州医学院附属医院肾内科,四川泸州646000

出　　处：《江西医学院学报》2005年第2期1-4,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的探讨金黄色葡萄球菌DNA对正常小鼠免疫功能的影响。方法纯化提取金黄色葡萄球菌DNA并注射于小鼠腹腔内,测定脾脏自然杀伤(NK)细胞、腹腔巨噬细胞(Mφ)的杀伤活性以及小鼠血清内IFNγ、TNFα的活性。结果实验组NK、Mφ细胞杀伤活性分别为:(75.0±13.9)%,(74.7±6.9)%;对照组分别为:(53.2±17.4)%,(53.8±8.8)%,两组数据各自比较差异均有显著性(P<0.01)。实验组IFNγ、TNFα的活性分别为120.33±45.32,28.77±8.51;对照组分别为125.25±27.57,25.40±9.68,两组数据各自比较差异无显著性意义(P>0.05)。结论金黄色葡萄球菌DNA可提高小鼠NK、Mφ细胞的活性,但对小鼠血清内IFNγ、TNFα的活性无显著影响。Objective To investigate the effects of staphylococcus aureus DNA on immunocompetence of mice . Methods The chromatic DNA purified from staphylococcus aureus was injected peritoneally into mice, and NK、Mφ cells and IFN-γ、TNF-α of activity were detected. Results The activity of NK、Mφ cells in experimental group were (75.0±13.9)%,(74.7±6.9)%, respectively. While they were (53.2±17.4)%, (53.8±8.8)% in control group respectively. They were significantly higher in experimental group than those in control group(P<0.01),The activity of IFN-γ、TNF-α in experimental group respectively were 120.33±45.32,28.77±8.51, and were 125.25±27.57,25.40±9.68 in control group respectively. They were no significantly higher in experimental group than those in control group(P>0.05)。Conclusion staphylococcus aureus DNA can augument NK、Mφ cells activity, but no effects on IFN-γ、TNF-α.

关 键 词：金黄色葡萄球菌DNA NK、Mφ细胞 IFN-Γ TNF-Α 动物 实验 小鼠 

分 类 号：R-33[医药卫生] R392