一株降解除草剂膦化麦黄桐(PPT)菌的筛选与鉴定  

ISOLATION AND IDENTIFICATION OF A BACTERIAL STRAIN FOR DEGRADING L -PHOSPHIOTHRICIN

作　　者：匡小婴[1] 饶志明[1] 沈微[1] 赵有玺[1] 方惠英[1] 诸葛健[1] 

机构地区：[1]江南大学工业微生物中心和工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《应用与环境生物学报》2005年第2期215-217,共3页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金 (No. 30300027 )资助~~

摘　　要：从喷洒有除草剂的土壤中分离到一株能分解膦化麦黄桐(PPT)的细菌.该菌在以PPT为唯一碳源的培养基上生长,能利用PPT的最高浓度为2. 7g/L.采用常规生理生化鉴定方法,并结合16SrDNA序列分析法对该菌进行鉴定.结果表明,该菌与生癌肠杆菌(Enterobactercancerogenus)序列相似性为99. 3%,在细菌分类学上属于肠杆菌.将它命名为Enterobactersp. PPT.One bacterial strain degrading L -phosphiothricin (PPT) was isolated from soil sprayed with herbicide. The strain could grow on medium containing PPT as sole carbon source and resist PPT with concentration up to 2.7 g/L. In addition to general physiological and biochemical properties, the strain was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain had similarity of 99.3% with that of Enterobacter cancerogenus , suggesting that the strain belong to Enterobacter . The strain was nominated as Enterobacter sp. PPT. Fig 4, Tab 2, Ref 7

关 键 词：膦化麦黄桐 生物降解菌 分离筛选 16s RDNA 

分 类 号：X172[环境科学与工程—环境科学]