一种新的好氧反硝化菌筛选方法的建立及新菌株的发现  

A NEW SCREENING METHOD FOR AEROBIC DENITRIFICATION BACTERIA AND ISOLATION OF A NOVEL STRAIN

作　　者：孔庆鑫[1] 李君文[1] 王新为[1] 金敏[1] 古长庆[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所

出　　处：《应用与环境生物学报》2005年第2期222-225,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家"八六三"计划项目(No. 2001AA214191; 2002AA601240 );天津市科技攻关重大项目 (No. 0231807111 )~~

摘　　要：利用间歇曝气富集,氰化钾(KCN)选择培养基筛选好氧反硝化的细菌,通过形态学特征、生理生化反应及16SrDNA同源性比较对筛得菌株进行鉴定,并对其好氧反硝化相关基因napA进行扩增并测序比较.筛选到一株可以柠檬酸钠为碳源,硝酸钾为氮源,进行好氧反硝化的细菌.在溶解氧(DO)为(9. 0±0. 5)mg/L的培养基中,该菌株5d内将硝态氮由282. 0mgL-1降解至149. 2mgL-1,其硝态氮去除率为46. 47ngmg-1 min-1,同时亚硝态氮仅有少量的积累.经鉴定,初步判定它为假单胞菌属,命名为Pseudomonassp.Y2-1-1.从其基因组中扩增出与好氧反硝化相关的周质硝酸盐还原酶(NAR)的亚基napA基因,并与已报道的napA基因进行Blast比较,发现具有较大差别.利用间歇曝气富集,氰化钾(KCN)选择培养基筛选好氧反硝化的细菌是非常有效的.初步认为Pseudomonassp.Y2 -1 -1是一株新的好氧反硝化菌.An aerobic bacterium Y2-1-1, enriched by intermittent aeration and screened with KCN screening culture medium, could be used for denitrification with sodium citrate as carbon resource and potassium nitrate as nitrogen resource. During 5 days, NO - 3 -N was decreased from 282.0 mg/L to 149.2 mg/L with low nitrite accumulation and the nitrate removal rate (NO - 3 -N) was 46.47 ng mg -1 min -1 . The strain was identified as Pseudomonas sp . Y2-1-1 according to its morphological, physiological and biochemical characters, as well as 16S rDNA sequence homology comparison. Its partial nap A gene was cloned and sequenced, and the gene was a subunit of periplasmic nitrate reductase (NAR), which was considered to be relative to aerobic denitrification, and found different from the reported nap A genes by Blast comparison. It was suggested that the Pseudomonas sp. Y2-1-1 be a novel aerobic denitrification bacterium. Fig 6, Tab 3, Ref 12

关 键 词：呼吸抑制剂 好氧反硝化 硝酸盐还原酶 NAPA 

分 类 号：X172[环境科学与工程—环境科学]