N^+注入选育益生菌及其产酶条件的研究  

Breeding of Probiotic Bacterium by N^+ Implantationand Optimization of Fermentation Condition

作　　者：程茂基[1] 陈丽娟[1] 蔡克周[1] 余增亮[2] 张束清[2] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]中国科学院等离子物理研究所,合肥230031

出　　处：《安徽农业大学学报》2005年第2期127-131,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(30100134)资助。

摘　　要：出发菌株黑曲霉AN01,经离子束多次诱变得变异菌株AN02。结果表明,出发菌株AN01酸性蛋白酶、纤维素酶和果胶酶的酶活分别由原来的1 298U·mL-1、2 551U·mL-1和5 620U·mL-1相继提高到14 252U·mL-1、13 016U·mL-1和13 206U·mL-1。变异菌株AN02经传5代培养,产酶特性稳定。该菌株的最佳产酶条件为在培养基基础营养为麸皮、豆粕和玉米芯的条件下,最佳无机氮源为硫酸铵,最佳pH为5 0,最佳含水量为60%,最佳发酵温度为30℃,最佳发酵周期为96h。A new strain Aspergillus niger AN02 was mutated from AN01 by N^+ implantation.The results showed that activities of acid-protease,cellulase and pectinase in Aspergillus niger were raised from 1 298 U·mL^(-1),2 551 U·mL^(-1) and 5 620 U·mL^(-1) to 14 252 U·mL^(-1),13 016 U·mL^(-1) and 13 206 U·mL^(-1) respectively.Characteristics of enzymes production in mutated strain Aspergillus niger AN02 kept stable by many times subculture.At the same time we study optimum conditions suitable for enzyme production in Aspergillus niger AN02,and the results demonstrated that enzymes activities in mutated strain Aspergillus niger were highest when the cultivate condition were as follows:fermentation temperature was 30℃,cultivate time was 96 hours,the water content in culture medium was 60 percent, pH was 5.0 and (NH_4)_2SO_4 was nitrogen source.

关 键 词：离子束 黑曲霉 诱变育种 复合酶 

分 类 号：S816.3[农业科学—饲料科学]