葡萄糖酸锌、MnCl_2对SiO_2致肺泡巨噬细胞NO、NOS水平变化的影响  

The Effect of Zinc Gluconate and Manganous Chloride on Change of Nitric Oxide and Nitric Oxide Synthesase Induced by Silica on Alveolar Macrophage

作　　者：周敏[1] 谈伟君[1] 焦石[1] 

机构地区：[1]东南大学公共卫生学院劳动卫生与环境卫生学系,江苏南京210009

出　　处：《环境与职业医学》2005年第2期125-126,129,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的]观察葡萄糖酸锌(ZnG)、氯化锰(MnCl2 )及其联合作用对二氧化硅(SiO2 )致肺泡巨噬细胞(AM)一氧化氮(NO)、一氧化氮合酶(NOS)水平变化的影响,寻找两者的最佳剂量组合。[方法]采用大鼠肺灌洗法纯化获得1×10 6/mlAM后按设计分为:①生理盐水对照组;②SiO2 组:含SiO2 2 0 0 μg/ml;③SiO2 +ZnG组:内含SiO2 2 0 0 μg/ml外,ZnG分别为1.0、1.5、2 .2 5 μg/ml;④SiO2 +MnCl2 组:内含SiO2 2 0 0 μg/ml外,MnCl2 分别为0 .2 5、0 .5、1.0 μg/ml;⑤ZnG、MnCl2 联合作用组:每组内含SiO2 2 0 0 μg/ml外,ZnG和MnCl2 各按上述3种剂量,采用两因素三水平正交设计,共设9个剂量组。3 7℃,5 %CO2 培养箱培养18h后,检测细胞内NO含量、NOS活性。[结果]与SiO2 组比,ZnG、MnCl2 可以显著降低肺泡巨噬细胞NO水平及NOS的活性(P <0 .0 1) ;ZnG和MnCl2 的交互作用具有统计学意义,1.5 μg/ml的ZnG与0 .2 5 μg/ml的MnCl2 联合作用效果最好,NO、NOS水平分别降为(1.44 0±0 .0 70 ) μmol/L、(0 .761±0 .10 1)U/ml。[结论]Zn、Mn及两者联合作用在体外可有效降低SiO2 粉尘致AM的NO、NOS水平,为Zn、Mn应用于矽肺的防治提供了实验依据。Objective] To study the effect of zinc gluconate and manganese chloride(solely and in combination) on the leves of nitric oxide and nitric oxide synthase in alveolar macrophages(AM) treated with silica dust,and find the optimal doses of zinc gluconate and manganese chloride in combination. [Methods] The experiment consist of five groups,there were control group,silicon dioxide,silicon dioxide and zinc gluconate,silicon dioxide and manganese chloride,zinc gluconate and manganese chloride.AM were treated with SiO 2 and corresponding tested chemicals,24 hours after,nitric oxide and nitric oxide synthase were measured in alveolar macrophages. [Results] Zinc gluconate and manganese chloride reduced nitric oxide and nitric oxide synthase significantly(P<0.01).The effects of the combination of two substance were stronger,the most suitable combination was the zinc gluconate at 1.5 μg/ml with manganese chloride at 0.25 μg/ml. [Conclusion] Zinc gluconate and manganese chloride solely and in combination can reduce nitric oxide and nitric oxide synthase in alveolar macrophages theated with SiO 2.

关 键 词：肺泡巨噬细胞 MnCl2 葡萄糖酸锌 水平变化 一氧化氮合酶(NOS) CO2培养箱 SiO2粉尘 NOS活性 mol/L 5μg 二氧化硅 最佳剂量 肺灌洗法 生理盐水 正交设计 NO含量 NO水平 作用效果 ML 合作 氯化锰 对照组 18h 细胞内 统计学 

分 类 号：R135.2[医药卫生—劳动卫生] R730.45[医药卫生—公共卫生与预防医学]